Rat Discs, Large Homolog 4 (DLG4) ELISA Kit | DLG4 elisa kit

Rat Discs, Large Homolog 4 (DLG4) ELISA Kit

Cat. Num. AAA20359

• Gene Names: DLG4; PSD95; SAP90; SAP-90
• Reactivity: Rat
• Synonyms: Discs; Large Homolog 4 (DLG4); Rat Discs; Large Homolog 4 (DLG4) ELISA Kit; PSD95; SAP90; Postsynaptic density protein 95; Synapse-associated protein 90; DLG4 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Discs, Large Homolog 4 (DLG4).; No significant cross-reactivity or interference between Discs, Large Homolog 4 (DLG4) and analogues was observed.
• Sequence Length: 767
• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.118ng/mL

• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Discs, Large Homolog 4 (DLG4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Discs, Large Homolog 4 (DLG4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for DLG4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Discs, Large Homolog 4 (DLG4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Discs, Large Homolog 4 (DLG4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Discs, Large Homolog 4 (DLG4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Discs, Large Homolog 4 (DLG4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for DLG4 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4557529
• NCBI GeneID: 1742
• NCBI Accession #: NP_001356.1
• NCBI GenBank Nucleotide #: NM_001365.3
• UniProt Accession #: P78352; Q92941; Q9UKK8; B7Z1S1; G5E939
• Molecular Weight: 80,125 Da
• NCBI Official Full Name: disks large homolog 4 isoform 1
• NCBI Official Synonym Full Names: discs, large homolog 4 (Drosophila)
• NCBI Official Symbol: DLG4
• NCBI Official Synonym Symbols: PSD95; SAP90; SAP-90
• NCBI Protein Information: disks large homolog 4
• UniProt Protein Name: Disks large homolog 4
• UniProt Gene Name: DLG4
• UniProt Synonym Gene Names: PSD95; PSD-95; SAP-90; SAP90
• UniProt Entry Name: DLG4_HUMAN