Rat dopamine, DA ELISA Kit | DA elisa kit
Rat dopamine, DA ELISA Kit
Reactivity
Rat
Synonyms
dopamine, DA; N/A; Rat dopamine, DA ELISA Kit; Rat dopamine; DA ELISA Kit; dopamine; DA; DA elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat DA. No significant cross-reactivity or interference between rat DA and analogues was observed.
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates
Detection Range
0.156 ng/ml-10 ng/ml
Sensitivity
< 0.039 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Related Product Information for DA elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DA bound in the initial step. The color development is stopped and the intensity of the color is measured.
