Rat E-Selectin/CD62e/SELE ELISA Kit | CD62e/SELE elisa kit

Rat E-Selectin/CD62e/SELE ELISA Kit

Cat. Num. AAA258499

• Reactivity: Rat
• Synonyms: E-Selectin/CD62e/SELE; N/A; Rat E-Selectin/CD62e/SELE ELISA Kit; E-Selectin ELISA Kit, Rat; selectin E; CD62e/SELE elisa kit

• Reactivity: Rat
• Specificity: For the quantitative determination of Rat E-Selectin/CD62e/SELE concentration in serum.
• Assay Type: Solid Phase Sandwich ELISA (quantitative)
• Sample Types: Serum
• Detection Range: 23.44-1500pg/mL
• Sensitivity: 3.78pg/mL
• Conjugation: HRP
• Materials Provided: 1. 96 well microplate coated with Capture Antibody; 2. Detection Antibody conjugated to HRP; 3. Standards; 4. Wash Buffer Concentrate; 5. Dilution Buffer Concentrate; 6. Color Reagent A; 7. Color Reagent B; 8. Stop Solution

Application Data

(This assay recognizes both recombinant and natural Rat SELE. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)

Standard Curve (Sample)

(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)

Related Product Information for CD62e/SELE elisa kit

This E-Selectin ELISA Kit, Rat is an enzyme-linked immunosorbent assay for the quantitative measurement of Rat E-Selectin protein in Serum. It contains recombinant Rat E-Selectin, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.

Background: E-selectin, also known as endothelial leukocyte adhesion molecule-1 (ELAM-1) and CD62E, is an inducible adhesion molecule that is expressed on the surfaces of stimulated vascular endothelial cells and is sometimes involved in cancer cell metastasis. E-selectin exhibits a complex mosaic structure consisting of a large extracellular region comprised of a lectin domain, an EGF-like domain, and a short consensus repeat (SCR) domain, followed by a transmembrane region and a relatively short (32 aa) cytoplasmic tail. As a member of the LEC-CAM or selectin family, E-selectin recognises and binds to sialylated carbohydrates including members of the Lewis X and Lewis A families found on monocytes, granulocytes, and T-lymphocytes. E-selectin supports rolling and stable arrest of leukocytes on activated vascular endothelium, and furthermore, it was indicated that it can also transduce an activating stimulus via the MAPK cascade into the endothelial cell during leukocyte adhesion. E-selectin regulates adhesive interactions between certain blood cells and endothelium. The soluble form of E selectin (sE-selectin) is a marker of endothelial activation, and has a potential role in the pathogenesis of cardiovascular disease as raised levels have been found in hypertension, diabetes and hyperlipidemia, although its association in established atherosclerosis disease and its value as a prognostic factor is more controversial. soluble E-selectin is inversely associated with the muscular component of the left ventricle, thereby suggesting that the lack of such a reparative factor may be associated with cardiac remodeling in end-stage renal disease (ESRD) patients. Besides, this adhesion molecule appears to be involved in the pathogenesis of atherosclerosis.

References

Roldn V, et al. (2003) Soluble E-selectin in cardiovascular disease and its risk factors. A review of the literature. Thromb Haemost. 90(6): 1007-20.
Kawase J, et al. (2009) Increase in E-selectin expression in umbilical vein endothelial cells by anticancer drugs and inhibition by cimetidine. Oncol Rep. 22(6): 1293-7.
Matsumoto K, et al. (2010) Soluble adhesion molecule E-selectin predicts cardiovascular events in Japanese patients with type 2 diabetes mellitus. Metabolism. 59(3): 320-4.
Stancanelli B, et al. (2010) Soluble e-selectin is an inverse and independent predictor of left ventricular wall thickness in end-stage renal disease patients. Nephron Clin Pract. 114(1): c74-80.