Rat Fatty-acid amide hydrolase 1 (FAAH) ELISA Kit | FAAH elisa kit

Rat Fatty-acid amide hydrolase 1 (FAAH) ELISA Kit

Cat. Num. AAA27236

• Gene Names: FAAH; PSAB; FAAH-1
• Reactivity: Rat
• Synonyms: Fatty-acid amide hydrolase 1 (FAAH); Rat Fatty-acid amide hydrolase 1 (FAAH) ELISA Kit; FAAH elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of FAAH1. No significant cross-reactivity or interference between FAAH1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FAAH1 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for FAAH elisa kit

Principle of the Assay: FAAH1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FAAH1 antibody and an FAAH1-HRP conjugate. The assay sample and buffer are incubated together with FAAH1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FAAH1 concentration since FAAH1 from samples and FAAH1-HRP conjugate compete for the anti-FAAH1 antibody binding site. Since the number of sites is limited, as more sites are occupied by FAAH1 from the sample, fewer sites are left to bind FAAH1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FAAH1 concentration in each sample is interpolated from this standard curve.

Intended Uses: This FAAH1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat FAAH1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

NCBI and Uniprot Product Information

• NCBI GI #: 166795287
• NCBI GeneID: 2166
• NCBI Accession #: NP_001432.2
• NCBI GenBank Nucleotide #: NM_001441.2
• Molecular Weight: 63,066 Da
• NCBI Official Full Name: fatty-acid amide hydrolase 1
• NCBI Official Synonym Full Names: fatty acid amide hydrolase
• NCBI Official Symbol: FAAH
• NCBI Official Synonym Symbols: PSAB; FAAH-1
• NCBI Protein Information: fatty-acid amide hydrolase 1; oleamide hydrolase 1; anandamide amidohydrolase 1
• UniProt Protein Name: Fatty-acid amide hydrolase 1
• UniProt Gene Name: FAAH
• UniProt Synonym Gene Names: FAAH1
• UniProt Entry Name: FAAH1_HUMAN