Rat GM-CSF ELISA Kit | GM-CSF elisa kit

Rat GM-CSF ELISA KIT

Cat. Num. AAA309919

• Gene Names: CSF2; GMCSF
• Reactivity: Rat
• Synonyms: GM-CSF; N/A; Rat GM-CSF ELISA KIT; GM-CSF elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant Rat GM-CSF Ligand
• Assay Type: Solid Phase Sandwich ELISA
• Samples: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Detection Range: 0.02-1.0 ng/mL
• Sensitivity: 7pg/mL

• Assay Time: 3 hour
• Sample Volume: 20 uL
• Detection Method: Colorimetric
• Preparation and Storage: Store at 4 degree C

Standard Curve (Sample)

(Representative standard curve for GM-CSF ELISA. GM-CSF was diluted in serial two-fold steps in Sample Diluent.)

Related Product Information for GM-CSF elisa kit

GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocytemacrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3-5). It shows clinical effectiveness in ameliorating chemotherapyinduced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled ahelices (8-10). Mature rat GM-CSF shares 56-69% amino acid sequence identity with canine, feline, human, mouse, and porcine GM-CSF. GM-CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF Ra/CD116 and the signal transducing common b chain (CD131) which is also a component of the highaffinity receptors for IL-3 and IL-5 (11, 12). In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF Ra (13). Rat GM-CSF is active on mouse cells, although mouse GM-CSF is only weakly active on rat cells (14, 15).

NCBI and Uniprot Product Information

• NCBI GI #: 183364
• NCBI GeneID: 1437
• Molecular Weight: 16,295 Da
• NCBI Official Full Name: GM-CSF
• NCBI Official Synonym Full Names: colony stimulating factor 2
• NCBI Official Symbol: CSF2
• NCBI Official Synonym Symbols: GMCSF
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor
• UniProt Gene Name: CSF2
• UniProt Synonym Gene Names: GMCSF; GM-CSF; CSF