Standard Curve (Sample)
Rat HLA-B Associated Transcript 3 (BAT3) ELISA Kit | BAT3 elisa kit
Rat HLA-B Associated Transcript 3 (BAT3) ELISA Kit
Gene Names
Bag6; Bat3
Reactivity
Rat
Synonyms
HLA-B Associated Transcript 3 (BAT3); N/A; Rat HLA-B Associated Transcript 3 (BAT3) ELISA Kit; BAG6; Protein G3; Protein Scythe; BCL2-associated athanogene 6; BAG family molecular chaperone regulator 6; Large proline-rich protein BAG6; BAT3 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of BAT3. No significant cross-reactivity or interference between BAT3 and analogues was observed.
Sequence Length
1146
Samples
Tissue homogenates, cell lysates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
<0.068ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BAT3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BAT3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for BAT3 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of BAT3 in rat tissue homogenates, cell lysates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BAT3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to BAT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BAT3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of BAT3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BAT3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to BAT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BAT3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of BAT3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
114,647 Da
NCBI Official Full Name
large proline-rich protein BAG6 isoform 1
NCBI Official Synonym Full Names
BCL2-associated athanogene 6
NCBI Official Symbol
Bag6
NCBI Official Synonym Symbols
Bat3
NCBI Protein Information
large proline-rich protein BAG6
UniProt Protein Name
Large proline-rich protein BAG6
UniProt Gene Name
Bag6
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Product Notes
The Rat BAT3 bag6 (Catalog #AAA68520) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA68520 ELISA Kit recognizes Rat BAT3. It is sometimes possible for the material contained within the vial of "HLA-B Associated Transcript 3 (BAT3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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