Standard Curve (Sample)
(Fig.1. Rat IFN-gamma Standard Curve.)
Rat IFN-gamma ELISA Kit | IFN-gamma elisa kit
Rat IFN-gamma ELISA Kit
Gene Names
Ifng; IFNG2
Reactivity
Rat
Applications
ELISA
Synonyms
IFN-gamma; N/A; Rat IFN-gamma ELISA Kit; IFG; IFI; IFN gamma; IFN Immune; IFNG; Interferon gamma; Interferon Gamma Precursor; Macrophage Activating Factor; MAF; T Cell Interferon; Type II Interferon; IFN-gamma elisa kit
Reactivity
Rat
Assay Type
Sandwich ELISA (quantitative)
Samples
Cell culture supernatants, Serum, Plasma, Other biological fluids
Detection Range
150pg/mL-2400pg/mL
Sensitivity
15pg/mL
Detection Method
Colorimetric
Assay Duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Features & Benefits
Rat IFN-gamma ELISA Kit has high sensitivity and excellent specificity for detection of Rat IFN-gamma. No significant cross-reactivity or interference between Rat IFN-gamma and analogues was observed.
Kit Components
Rat IFN-gamma microplate
Rat IFN-gamma standard
Rat IFN-gamma detect antibody
Standard diluent
Sample diluent
Chromogen solution A
Chromogen solution B
Stop solution
Wash buffer
Plate covers.
Rat IFN-gamma standard
Rat IFN-gamma detect antibody
Standard diluent
Sample diluent
Chromogen solution A
Chromogen solution B
Stop solution
Wash buffer
Plate covers.
Usage Notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Applicable Applications for IFN-gamma elisa kit
ELISA
Notes
Rat Interferon Gamma (IFN-gamma) ELISA Kit employs a two-site sandwich ELISA to quantitate IFN-gamma in samples. An antibody specific for IFN-gamma has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-gamma present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Rat IFN-gamma detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of IFN-gamma bound in the initial step. The color development is stopped and the intensity of the color is measured.
Preparation and Storage
The unopened kit should be stored at 2 - 8 degree C. After opening, please store refer to protocols.
Standard Curve (Sample)
(Fig.1. Rat IFN-gamma Standard Curve.)
Standard Curve (Sample)
(Fig.1. Rat IFN-gamma Standard Curve.)
Related Product Information for IFN-gamma elisa kit
Description: Rat IFN-gamma ELISA Kit employs a two-site sandwich ELISA to quantitate Rat IFN-gamma.
Background: IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.
Background: IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.
Product Categories/Family for IFN-gamma elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,918 Da
NCBI Official Full Name
interferon gamma
NCBI Official Synonym Full Names
interferon gamma
NCBI Official Symbol
Ifng
NCBI Official Synonym Symbols
IFNG2
NCBI Protein Information
interferon gamma; IFN-gamma
UniProt Protein Name
Interferon gamma
UniProt Gene Name
Ifng
UniProt Synonym Gene Names
IFN-gamma
UniProt Entry Name
IFNG_RAT
Similar Products
Product Notes
The Rat IFN-gamma ifng (Catalog #AAA332580) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332580 ELISA Kit recognizes Rat IFN-gamma. AAA Biotech's IFN-gamma can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the IFN-gamma ifng for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFN-gamma, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.