Rat Insulin (INS) ELISA Kit | INS elisa kit

Rat Insulin (INS) ELISA Kit

Cat. Num. AAA22797

• Gene Names: INS; IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10
• Reactivity: Rat
• Synonyms: Insulin (INS); N/A; Rat Insulin (INS) ELISA Kit; INS elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of insulin. No significant cross-reactivity or interference between insulin and analogues was observed.
• Sequence Length: 110
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Quantitative Competitive
• Detection Range: 123.5-10,000pg/mL
• Sensitivity: < 51.6pg/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level insulin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level insulin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for INS elisa kit

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of insulin in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to insulin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled insulin and unlabeled insulin (Standards or samples) with the pre-coated antibody specific to insulin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of insulin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of insulin in the sample.

References

Astaxanthin Ameliorates high-fat diet-induced cardiac damage and fibrosis by upregulating and activating SIRT1; Saudi J Biol Sci; 2021 Dec;28(12):7012-7021.; PMID: 34867002; Abdullah S Shatoor; Department of Medicine, Cardiology Section, College of Medicine, King Khalid University (KKU), Abha, Saudi Arabia.

NCBI and Uniprot Product Information

• NCBI GI #: 386828
• NCBI GeneID: 3630
• UniProt Accession #: P01308; Q5EEX2
• Molecular Weight: 21,537 Da
• NCBI Official Full Name: insulin
• NCBI Official Synonym Full Names: insulin
• NCBI Official Symbol: INS
• NCBI Official Synonym Symbols: IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10
• NCBI Protein Information: insulin
• UniProt Protein Name: Insulin
• UniProt Gene Name: INS