Rat IP-10 ELISA Kit | IP-10 elisa kit

IP-10 (Rat) OmniKine ELISA Kit

Cat. Num. AAA315699

• Gene Names: Cxcl10; IP-10; Scyb10
• Reactivity: Rat
• Synonyms: IP-10; N/A; IP-10 (Rat) OmniKine ELISA Kit; C-X-C motif chemokine 10; 10 kDa interferon gamma-induced protein; Interferon-inducible protein 10; Protein Mob-1; Small-inducible cytokine B10; IP-10 elisa kit

• Reactivity: Rat
• Specificity: The OmniKine™ Rat IP-10 ELISA is capable of recognizing both recombinant and naturally produced Rat IP-10 proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 2% cross reactivity.
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 98
• Assay Type: Quantitative Sandwich
• Samples: Cell Lysates, Sera and Plasma

• Preparation and Storage: Store entire kit at 4 degree C until use.

Standard Curve (Sample)

Related Product Information for IP-10 elisa kit

Background: Rat IP-10 or 10 kDa Interferon-gamma Induced Protein, also known as C-XC Motif Chemokine 10, is a 98 amino acid cytokine protein encoded by the Cxcl10 gene located at locus 14p22 on chromosome 14. After initial synthesis, the 21 residue signal sequence is removed from the N-terminal region, allowing the 77 residue IP-10 protein to fold correctly and mature. IP-10 is known to be a proinflammatory cytokine that can induce DNA synthesis, cell proliferation and cell migration in vascular smooth muscle cells. In addition to its role as a proinflammatory cytokine, may participate in T-cell effector function and perhaps T-cell development via induction by oncogenic Ras and serum growth factors; the oncogenic induction is constitutive whereas the serum induction is transient.

Principle of the Assay: The OmniKine™ Rat IP-10 ELISA Kit contains the components necessary for quantitative determination of natural orrecombinant Rat IP-10 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) isbound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Rat IP-10 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of nonspecific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.

NCBI and Uniprot Product Information

• NCBI GI #: 20806125
• NCBI GeneID: 245920
• NCBI Accession #: NP_620789.1
• NCBI GenBank Nucleotide #: NM_139089.1
• UniProt Accession #: P48973; P70538; Q6GTC7
• Molecular Weight: 10,729 Da
• NCBI Official Full Name: C-X-C motif chemokine 10
• NCBI Official Synonym Full Names: C-X-C motif chemokine ligand 10
• NCBI Official Symbol: Cxcl10
• NCBI Official Synonym Symbols: IP-10; Scyb10
• NCBI Protein Information: C-X-C motif chemokine 10
• UniProt Protein Name: C-X-C motif chemokine 10
• UniProt Gene Name: Cxcl10
• UniProt Synonym Gene Names: Inp10; Mob1; Scyb10; Gamma-IP10; IP-10