Standard Curve (Sample)
(Fig.1. Rat Mitochondrial import receptor subunit TOM20 homolog (TOMM20) Standard Curve.)
Rat Mitochondrial Import Receptor Subunit TOM20 Homolog (TOMM20) ELISA Kit | TOMM20 elisa kit
Rat Mitochondrial Import Receptor Subunit TOM20 Homolog (TOMM20) ELISA Kit
Reactivity
Rat
Synonyms
Mitochondrial Import Receptor Subunit TOM20 Homolog (TOMM20); N/A; Rat Mitochondrial Import Receptor Subunit TOM20 Homolog (TOMM20) ELISA Kit; RP4-597N16.2; KIAA0016; MAS20; MGC117367; MOM19; TOM20; translocase of outer mitochondrial membrane 20 homolog; translocase of outer mitochondrial membrane 20 homolog type; TOMM20 elisa kit
Reactivity
Rat
Specificity
Rat Mitochondrial import receptor subunit TOM20 homolog (TOMM20) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TOMM20. No significant cross-reactivity or interference between Rat TOMM20 and analogues was observed.
Sequence Length
145
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Rat Mitochondrial import receptor subunit TOM20 homolog (TOMM20) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Rat Mitochondrial import receptor subunit TOM20 homolog (TOMM20) Standard Curve.)
Related Product Information for TOMM20 elisa kit
Description: This Rat Mitochondrial import receptor subunit TOM20 homolog (TOMM20) ELISA Kit employs a two-site sandwich ELISA to quantitate TOMM20 in samples. An antibody specific for TOMM20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TOMM20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOMM20 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOMM20 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TOMM20L Belongs to the Tom20 family.Tom20 is the master receptor for protein import into mitochondria. Analysis of motifs present in Tom20 sequences from fungi and animals found several highly conserved regions, including features of the transmembrane segment, the ligand-binding domain and functionally important flexible segments at the N terminus and the C terminus of the protein. Hidden Markov model searches of genome sequence data revealed novel isoforms of Tom20 in vertebrate and invertebrate animals. A three-dimensional comparative model of the novel type I Tom20, based on the structurally characterized type II isoform, shows important differences in the amino acid residues lining the ligand-binding groove, where the type I protein from animals is more similar to the fungal form of Tom20.
Background: TOMM20L Belongs to the Tom20 family.Tom20 is the master receptor for protein import into mitochondria. Analysis of motifs present in Tom20 sequences from fungi and animals found several highly conserved regions, including features of the transmembrane segment, the ligand-binding domain and functionally important flexible segments at the N terminus and the C terminus of the protein. Hidden Markov model searches of genome sequence data revealed novel isoforms of Tom20 in vertebrate and invertebrate animals. A three-dimensional comparative model of the novel type I Tom20, based on the structurally characterized type II isoform, shows important differences in the amino acid residues lining the ligand-binding groove, where the type I protein from animals is more similar to the fungal form of Tom20.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mitochondrial import receptor subunit TOM20 homolog
NCBI Official Synonym Full Names
translocase of outer mitochondrial membrane 20
NCBI Official Symbol
Tomm20
NCBI Protein Information
mitochondrial import receptor subunit TOM20 homolog
UniProt Protein Name
Mitochondrial import receptor subunit TOM20 homolog
UniProt Gene Name
Tomm20
UniProt Entry Name
TOM20_RAT
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Product Notes
The Rat TOMM20 tomm20 (Catalog #AAA330181) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330181 ELISA Kit recognizes Rat TOMM20. It is sometimes possible for the material contained within the vial of "Mitochondrial Import Receptor Subunit TOM20 Homolog (TOMM20), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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