Rat Neuron-specific enolase (NSE) ELISA Kit | NSE elisa kit
Rat Neuron-specific enolase (NSE) ELISA Kit
Reactivity
Rat
Synonyms
Neuron-specific enolase (NSE); N/A; Rat Neuron-specific enolase (NSE) ELISA Kit; NSE elisa kit
Reactivity
Rat
Sequence Length
434
Assay Type
Sandwich
Detection Range
20 ng/mL-0.312 ng/mL
Sensitivity
Up to 0.06 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for NSE elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat NSE monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
47,266 Da
NCBI Official Full Name
neuron-specific enolase
UniProt Protein Name
Neuron-specific enolase
UniProt Gene Name
NSE
UniProt Entry Name
G9JNA8_CAPHI
