Rat NGF beta ELISA Kit | NGF elisa kit

Rat NGF beta ELISA Kit

Cat. Num. AAA105783

• Gene Names: Ngf; Ngfb; beta-NGF
• Reactivity: Rat
• Applications: ELISA
• Synonyms: NGF beta; N/A; Rat NGF beta ELISA Kit; NGFB; Beta-nerve growth factor; Beta-NGF; NGF elisa kit

• Reactivity: Rat
• Specificity: The Rat IL-6 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat IL-6 proteins within the range of 62.5 pg/ml-4000 pg/ml.
• Sequence Length: 241
• Samples: Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates
• Sensitivity: 30 pg/ml.

• Applicable Applications for NGF elisa kit: ELISA (Sandwich ELISA)
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Standard Curve (Sample)

Related Product Information for NGF elisa kit

Principle of the Assay: The AAA Biotech's Rat IL-6 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-6 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Rat IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm!!Background/Introduction: Interleukin 6 is a multifunctional cytokine that plays important roles in host defense, acute phase reactions, immune responses, nerve cell functions and hematopoiesis. IL-6 is a prototypic member of the IL-6 superfamily of cytokines that share gp130 as a component required for signal transduction. The rat, mouse and human IL-6 cDNAs have been cloned. Rat IL-6 cDNA encodes a 211 amino acid residue precursor polypeptide with a hydrophobic signal peptide that is cleaved to generate the 187 aa residue mature protein. At the protein sequence level, there is approximately 39% identity between rat and human, and 87% identity between mouse and rat IL-6. IL-6 is expressed by a variety of normal and transformed lymphoid and non-lymphoid cells. The production of IL-6 is up-regulated by numerous signals such as mitogenic or antigenic stimulation, lipopolysaccharides, calcium ionophores, cytokines and viruses. IL-4, IL-10 and IL-13 inhibit IL-6 expression in monocytes. Elevated serum IL-6 levels have been observed in a number of pathological conditions, including bacterial and viral infections,trauma, autoimmune diseases, inflammations and malignancies.

NCBI and Uniprot Product Information

• NCBI GI #: 456367246
• NCBI GeneID: 310738
• NCBI Accession #: NP_001263984.1
• NCBI GenBank Nucleotide #: NM_001277055.1
• UniProt Accession #: P25427
• Molecular Weight: 27,010 Da
• NCBI Official Full Name: beta-nerve growth factor
• NCBI Official Synonym Full Names: nerve growth factor (beta polypeptide)
• NCBI Official Symbol: Ngf
• NCBI Official Synonym Symbols: Ngfb; beta-NGF
• NCBI Protein Information: beta-nerve growth factor
• UniProt Protein Name: Beta-nerve growth factor
• UniProt Gene Name: Ngf
• UniProt Synonym Gene Names: Ngfb; Beta-NGF
• UniProt Entry Name: NGF_RAT