Reactivity
Rat
Sequence Length
638
Assay Type
Sandwich
Detection Range
20 ng/mL-0.312 ng/mL
Sensitivity
Up to 0.06 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for PK elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat PK monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
71,370 Da
NCBI Official Full Name
plasma kallikrein preproprotein
NCBI Official Synonym Full Names
kallikrein B, plasma (Fletcher factor) 1
NCBI Official Symbol
KLKB1
NCBI Official Synonym Symbols
PPK; KLK3
NCBI Protein Information
plasma kallikrein; kininogenin; Fletcher factor; plasma prekallikrein; plasma kallikrein heavy chain; plasma kallikrein light chain
UniProt Protein Name
Plasma kallikrein
UniProt Gene Name
KLKB1
UniProt Synonym Gene Names
KLK3
UniProt Entry Name
KLKB1_HUMAN