Rat Protein S (PROS) ELISA Kit | PROS elisa kit

Rat Protein S (PROS) ELISA Kit

Cat. Num. AAA189770

• Reactivity: Rat
• Synonyms: Protein S (PROS); N/A; Rat Protein S (PROS) ELISA Kit; PSA; PS; PS26; PS21; PS22; PS23; PS24; PS25; VKDPS; VKDP-S; Protein Sa; Vitamin K Dependent Protein S; PROS/Protein S; PROS elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of PROS. No significant cross-reactivity or interference between PROS and analogues was observed.
• Assay Type: Quantitative Sandwich
• Samples: Rat plasma
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.134ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PROS were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PROS were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Store at 4 degree C for 1 month/-20 degree C for long-term (within shelf life); Shelf Life: 8 months; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for PROS elisa kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PROS. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PROS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PROS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PROS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PROS in rat plasma.

Product Categories/Family for PROS elisa kit

Metabolic pathway; Hematology

NCBI and Uniprot Product Information

• NCBI GI #: 1709810
• NCBI GeneID: 81750
• UniProt Accession #: P53813
• Molecular Weight: 73.9 kDa
• NCBI Official Full Name: Vitamin K-dependent protein S
• NCBI Official Synonym Full Names: protein S (alpha)
• NCBI Official Symbol: Pros1
• NCBI Protein Information: vitamin K-dependent protein S
• UniProt Protein Name: Vitamin K-dependent protein S
• UniProt Gene Name: Pros1
• UniProt Synonym Gene Names: Pros
• UniProt Entry Name: PROS_RAT