Standard Curve (Sample)
Rat proteinkinaseC (PKC) ELISA Kit | PKC elisa kit
Rat proteinkinaseC (PKC) ELISA Kit
Gene Names
Prkcg; PKC; PKCI; Prkc; Prkcc; RATPKCI
Reactivity
Rat
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigen,specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
proteinkinaseC (PKC); N/A; Rat proteinkinaseC (PKC) ELISA Kit; PKC elisa kit
Reactivity
Rat
Specificity
No cross-reaction with other factors.
Purity/Purification
Antigen,specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
0.35mg/ml (varies by lot)
Samples
Rat Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Picp Concentration
Assay Type
Sandwich
Detection Range
50 ng/ml-0.78 ng/ml
Sensitivity
0.08 ng/ml.
Applicable Applications for PKC elisa kit
IP (Immunoprecipitation), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for PKC elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat PICP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
protein kinase C gamma type
NCBI Official Synonym Full Names
protein kinase C, gamma
NCBI Official Symbol
Prkcg
NCBI Official Synonym Symbols
PKC; PKCI; Prkc; Prkcc; RATPKCI
NCBI Protein Information
protein kinase C gamma type; PKC-gamma; protein kinase C type I (gamma type)
UniProt Protein Name
Protein kinase C gamma type
UniProt Gene Name
Prkcg
UniProt Synonym Gene Names
Pkcc; Pkcg; Prkcc; PKC-gamma
UniProt Entry Name
KPCG_RAT
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Product Notes
The Rat PKC prkcg (Catalog #AAA54200) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA54200 ELISA Kit recognizes Rat PKC. AAA Biotech's proteinkinaseC (PKC) can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PKC prkcg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "proteinkinaseC (PKC), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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