Reactivity
Rat
Sequence Length
254
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids
Detection Range
0.05-30ng/ml
Sensitivity
0.024ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Related Product Information for SRD5A2 elisa kit
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat SRD5A2 antibody. SRD5A2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat SRD5A2 Antibody is added and binds to SRD5A2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SRD5A2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat SRD5A2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Steroid 5-Alpha-Reductase 2 (also known as SRD5A2) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Steroid 5-Alpha-Reductase 2 (also known as SRD5A2) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
28,393 Da
NCBI Official Full Name
SRD5A2 protein
NCBI Official Synonym Full Names
steroid 5 alpha-reductase 2
NCBI Official Symbol
SRD5A2
NCBI Protein Information
3-oxo-5-alpha-steroid 4-dehydrogenase 2
UniProt Protein Name
3-oxo-5-alpha-steroid 4-dehydrogenase 2
UniProt Gene Name
SRD5A2
UniProt Synonym Gene Names
S5AR 2