Rat Stromal Cell Derived Factor 1 (SDF1) ELISA Kit | SDF1 elisa kit

Rat Stromal Cell Derived Factor 1 (SDF1) High Sensitive ELISA Kit

Cat. Num. AAA188482

• Gene Names: Cxcl12; Sdf1
• Reactivity: Rat
• Applications: ELISA
• Synonyms: Stromal Cell Derived Factor 1 (SDF1); N/A; Rat Stromal Cell Derived Factor 1 (SDF1) High Sensitive ELISA Kit; Stromal Cell Derived Factor 1; CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-Stimulating Factor; Chemokine C-X-C-Motif Ligand 12; SDF1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Stromal Cell Derived Factor 1 (SDF1).; No significant cross-reactivity or interference between High Sensitive Stromal Cell Derived Factor 1 (SDF1) and analogues was observed.
• Sequence Length: 89
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 6.2pg/mL

• Applicable Applications for SDF1 elisa kit: ELISA
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Stromal Cell Derived Factor 1 (SDF1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Stromal Cell Derived Factor 1 (SDF1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Stromal Cell Derived Factor 1 (SDF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Stromal Cell Derived Factor 1 (SDF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Stromal Cell Derived Factor 1 (SDF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Stromal Cell Derived Factor 1 (SDF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

NCBI and Uniprot Product Information

• NCBI GI #: 11559933
• NCBI GeneID: 24772
• NCBI Accession #: NP_071513.1
• NCBI GenBank Nucleotide #: NP_071513.1
• UniProt Accession #: Q9QZD1
• NCBI Official Full Name: stromal cell-derived factor 1 isoform alpha
• NCBI Official Synonym Full Names: C-X-C motif chemokine ligand 12
• NCBI Official Symbol: Cxcl12
• NCBI Official Synonym Symbols: Sdf1
• NCBI Protein Information: stromal cell-derived factor 1
• UniProt Protein Name: Chemokine (C-X-C motif) ligand 12 (Stromal cell-derived factor 1)
• UniProt Gene Name: Cxcl12
• UniProt Entry Name: Q9QZD1_RAT