tartrate-resistant acid phosphatase 5b, TRACP-5b ELISA Kit | TRACP-5b elisa kit
Rat tartrate-resistant acid phosphatase 5b, TRACP-5b ELISA Kit
Reactivity
Rat
Synonyms
tartrate-resistant acid phosphatase 5b, TRACP-5b; N/A; Rat tartrate-resistant acid phosphatase 5b, TRACP-5b ELISA Kit; Rat tartrate-resistant acid phosphatase 5b; TRACP-5b ELISA Kit; tartrate-resistant acid phosphatase 5b; TRACP-5b; TRACP-5b elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat TRACP-5b. No significant cross-reactivity or interference between rat TRACP-5b and analogues was observed.
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates
Detection Range
0.312 mIU/ml-20 mIU/ml
Sensitivity
< 0.078 mIU/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for TRACP-5b elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TRACP-5b has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRACP-5b present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRACP-5b is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRACP-5b bound in the initial step. The color development is stopped and the intensity of the color is measured.