Standard Curve (Sample)
Rat Thromboxane A2 (TXA2) ELISA Kit | TXA2 elisa kit
Rat Thromboxane A2 (TXA2) ELISA Kit
Reactivity
Rat
Synonyms
Thromboxane A2 (TXA2); N/A; Rat Thromboxane A2 (TXA2) ELISA Kit; TXA2 elisa kit
Reactivity
Rat
Specificity
Rat Thromboxane A2 (TXA2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TXA2. No significant cross-reactivity or interference between Rat TXA2 and analogues was observed.
Assay Type
Sandwich
Samples
Rat serum, cell culture supernates and other biological fluids
Detection Range
30 ng/L - 480 ng/L
Sensitivity
< 1 ng/L
Intra-assay Precision
Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TXA2 elisa kit
Background: Thromboxane B2 is a metabolite of thromboxane A2 which is known to be highly unstable under physiological conditions. Thromboxane B2 is recognized as a valuable substance for study of a variety of biochemical processes as well as important synthetic precursor to thromboxane A2.Several syntheses of thromboxane B2 have been completed, either in racemic form or deriving the stereocenters from a chiral pool. The Burke group is currently endeavoring to complete the total synthesis of thromboxane B2. Thromboxane B2 is an inactive metabolite/product of thromboxane A2. It is almost completely cleared in the urine.It itself is not involved in platelet activation and aggregation in case of a wound, but its precursor, thromboxane A2.
Principle of the Assay: Rat Thromboxane A2 (TXA2) ELISA Kit employs a two-site sandwich ELISA to quantitate TXA2 in samples. An antibody specific for TXA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TXA2 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Rat TXA2 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of TXA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: Rat Thromboxane A2 (TXA2) ELISA Kit employs a two-site sandwich ELISA to quantitate TXA2 in samples. An antibody specific for TXA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TXA2 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Rat TXA2 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of TXA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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Product Notes
The Rat TXA2 (Catalog #AAA330161) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330161 ELISA Kit recognizes Rat TXA2. It is sometimes possible for the material contained within the vial of "Thromboxane A2 (TXA2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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