Standard Curve (Sample)
(Fig.1. Rat Transient receptor potential cation channel subfamily M member 4 (TRPM4) Standard Curve.)
Rat Transient Receptor Potential Cation Channel Subfamily M Member 4 (TRPM4) ELISA Kit | TRPM4 elisa kit
Rat Transient Receptor Potential Cation Channel Subfamily M Member 4 (TRPM4) ELISA Kit
Gene Names
Trpm4; Mls2s; LTrpC-4
Reactivity
Rat
Synonyms
Transient Receptor Potential Cation Channel Subfamily M Member 4 (TRPM4); N/A; Rat Transient Receptor Potential Cation Channel Subfamily M Member 4 (TRPM4) ELISA Kit; TRPM4; FLJ20041; TRPM4B; TRPM4 elisa kit
Reactivity
Rat
Specificity
Rat Transient receptor potential cation channel subfamily M member 4 (TRPM4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TRPM4. No significant cross-reactivity or interference between Rat TRPM4 and analogues was observed.
Sequence Length
1208
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Rat Transient receptor potential cation channel subfamily M member 4 (TRPM4) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Rat Transient receptor potential cation channel subfamily M member 4 (TRPM4) Standard Curve.)
Related Product Information for TRPM4 elisa kit
Description: This Rat Transient receptor potential cation channel subfamily M member 4 (TRPM4) ELISA Kit employs a two-site sandwich ELISA to quantitate TRPM4 in samples. An antibody specific for TRPM4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRPM4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRPM4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRPM4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TRPM5 expression in several human and rodent cell lines, including murine neuronal cells, Burkitt lymphoma cells, murine B-lymphoma cells, HeLa cells, murine pancreatic beta cells, rat beta cells, and human pancreatic islets. No endogenous TRPM5 was detected in human embryonic kidney (HEK)-293 cells. Among the differentially expressed clones, one encoded Trpm5. In situ hybridization detected Trpm5 expression restricted to mouse taste tissue. In taste cells, Trpm5 was coexpressed with taste-signaling molecules such as alpha-gustducin, G protein gamma-13 subunit, phospholipase C-beta-2 (PLCB2), and inositol 1,4,5-trisphosphate receptor type III.
Background: TRPM5 expression in several human and rodent cell lines, including murine neuronal cells, Burkitt lymphoma cells, murine B-lymphoma cells, HeLa cells, murine pancreatic beta cells, rat beta cells, and human pancreatic islets. No endogenous TRPM5 was detected in human embryonic kidney (HEK)-293 cells. Among the differentially expressed clones, one encoded Trpm5. In situ hybridization detected Trpm5 expression restricted to mouse taste tissue. In taste cells, Trpm5 was coexpressed with taste-signaling molecules such as alpha-gustducin, G protein gamma-13 subunit, phospholipase C-beta-2 (PLCB2), and inositol 1,4,5-trisphosphate receptor type III.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
transient receptor potential cation channel subfamily M member 4
NCBI Official Synonym Full Names
transient receptor potential cation channel, subfamily M, member 4
NCBI Official Symbol
Trpm4
NCBI Official Synonym Symbols
Mls2s; LTrpC-4
NCBI Protein Information
transient receptor potential cation channel subfamily M member 4
UniProt Protein Name
Transient receptor potential cation channel subfamily M member 4
UniProt Gene Name
Trpm4
UniProt Synonym Gene Names
Ltrpc4; LTrpC-4; LTrpC4
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Product Notes
The Rat TRPM4 trpm4 (Catalog #AAA330168) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330168 ELISA Kit recognizes Rat TRPM4. It is sometimes possible for the material contained within the vial of "Transient Receptor Potential Cation Channel Subfamily M Member 4 (TRPM4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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