Standard Curve (Sample)
(Fig.1. Rat Uroporphyrinogen decarboxylase (UROD) Standard Curve.)
Rat Uroporphyrinogen Decarboxylase (UROD) ELISA Kit | UROD elisa kit
Rat Uroporphyrinogen Decarboxylase (UROD) ELISA Kit
Gene Names
Urod; porphyrinogen carboxy-lyase
Reactivity
Rat
Synonyms
Uroporphyrinogen Decarboxylase (UROD); N/A; Rat Uroporphyrinogen Decarboxylase (UROD) ELISA Kit; UROD; RP11-69J16.2; PCT; fifth enzyme of heme biosynthetic pathway; fifth enzyme of the heme biosynthetic pathway; uroporphyrinogenI decarboxylase; UROD elisa kit
Reactivity
Rat
Specificity
Rat Uroporphyrinogen decarboxylase (UROD) ELISA Kit has high sensitivity and excellent specificity for detection of Rat UROD. No significant cross-reactivity or interference between Rat UROD and analogues was observed.
Sequence Length
364
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Rat Uroporphyrinogen decarboxylase (UROD) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Rat Uroporphyrinogen decarboxylase (UROD) Standard Curve.)
Related Product Information for UROD elisa kit
Description: This Rat Uroporphyrinogen decarboxylase (UROD) ELISA Kit employs a two-site sandwich ELISA to quantitate UROD in samples. An antibody specific for UROD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UROD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UROD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UROD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: URODencodes the fifth enzyme of the heme biosynthetic pathway. This enzyme is responsible for catalyzing the conversion of uroporphyrinogen to coproporphyrinogen through the removal of four carboxymethyl side chains.Uroporphyrinogen III decarboxylase (UroD) is a homodimeric enzyme which catalyzes the fifth step in heme biosynthesis: the elimination of carboxyl groups from the four acetate side chains of uroporphyrinogen III to yield coproporphyrinogen III. At low substrate concentrations the reaction is believed to follow an ordered route, with the sequential removal of CO2 from the D, A, B, and C rings, whereas at higher substrate/enzyme levels a random route seems to be operative. The enzyme functions as a dimer in solution, and both the enzymes from human and tobacco have been crystallized and solved at good resolutions.
Background: URODencodes the fifth enzyme of the heme biosynthetic pathway. This enzyme is responsible for catalyzing the conversion of uroporphyrinogen to coproporphyrinogen through the removal of four carboxymethyl side chains.Uroporphyrinogen III decarboxylase (UroD) is a homodimeric enzyme which catalyzes the fifth step in heme biosynthesis: the elimination of carboxyl groups from the four acetate side chains of uroporphyrinogen III to yield coproporphyrinogen III. At low substrate concentrations the reaction is believed to follow an ordered route, with the sequential removal of CO2 from the D, A, B, and C rings, whereas at higher substrate/enzyme levels a random route seems to be operative. The enzyme functions as a dimer in solution, and both the enzymes from human and tobacco have been crystallized and solved at good resolutions.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Uroporphyrinogen decarboxylase
NCBI Official Synonym Full Names
uroporphyrinogen decarboxylase
NCBI Official Symbol
Urod
NCBI Official Synonym Symbols
porphyrinogen carboxy-lyase
NCBI Protein Information
uroporphyrinogen decarboxylase
UniProt Protein Name
Uroporphyrinogen decarboxylase
UniProt Gene Name
Urod
UniProt Synonym Gene Names
UPD; URO-D
Similar Products
Product Notes
The Rat UROD urod (Catalog #AAA330156) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330156 ELISA Kit recognizes Rat UROD. It is sometimes possible for the material contained within the vial of "Uroporphyrinogen Decarboxylase (UROD), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.