Standard Curve (Sample)
(Fig.1. Rat Very low-density lipoprotein receptor (VLDLR) Standard Curve.)
Rat Very Low-Density Lipoprotein Receptor (VLDLR) ELISA Kit | VLDLR elisa kit
Rat Very Low-Density Lipoprotein Receptor (VLDLR) ELISA Kit
Reactivity
Rat
Synonyms
Very Low-Density Lipoprotein Receptor (VLDLR); N/A; Rat Very Low-Density Lipoprotein Receptor (VLDLR) ELISA Kit; VLDLR; RP11-320E16.1; CHRMQ1; FLJ35024; VLDLRCH; VLDLR elisa kit
Reactivity
Rat
Specificity
Rat Very low-density lipoprotein receptor (VLDLR) ELISA Kit has high sensitivity and excellent specificity for detection of Rat VLDLR. No significant cross-reactivity or interference between Rat VLDLR and analogues was observed.
Sequence Length
873
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Rat Very low-density lipoprotein receptor (VLDLR) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Rat Very low-density lipoprotein receptor (VLDLR) Standard Curve.)
Related Product Information for VLDLR elisa kit
Description: This Rat Very low-density lipoprotein receptor (VLDLR) ELISA Kit employs a two-site sandwich ELISA to quantitate VLDLR in samples. An antibody specific for VLDLR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VLDLR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VLDLR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VLDLR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Sugie et al. (2005) classified XMEA as a form of autophagic vacuolar myopathy, characterized by intracytoplasmic autophagic vacuoles with sarcolemmal features.The clinical course was mild; the patients suffered from slowly progressive muscle weakness mainly in the legs, but did not lose their ability to walk. There was no evidence of cardiac or neural involvement. Serum creatine kinase was elevated. By electron microscopy, an excessive number of autophagic vacuoles with staining properties of lysosomes were observed. The granular and membranous material contained in these vacuoles was actively exocytosed. The authors suggested that this disorder differed from the muscular dystrophy of Duchenne and Becker and of Emery-Dreifuss as well as from X-linked myotubular myopathy.
Background: Sugie et al. (2005) classified XMEA as a form of autophagic vacuolar myopathy, characterized by intracytoplasmic autophagic vacuoles with sarcolemmal features.The clinical course was mild; the patients suffered from slowly progressive muscle weakness mainly in the legs, but did not lose their ability to walk. There was no evidence of cardiac or neural involvement. Serum creatine kinase was elevated. By electron microscopy, an excessive number of autophagic vacuoles with staining properties of lysosomes were observed. The granular and membranous material contained in these vacuoles was actively exocytosed. The authors suggested that this disorder differed from the muscular dystrophy of Duchenne and Becker and of Emery-Dreifuss as well as from X-linked myotubular myopathy.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
very low-density lipoprotein receptor
NCBI Official Symbol
VLDLR
NCBI Protein Information
very low-density lipoprotein receptor
UniProt Protein Name
Very low-density lipoprotein receptor
UniProt Gene Name
VLDLR
UniProt Synonym Gene Names
VLDL receptor; VLDL-R
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Product Notes
The Rat VLDLR vldlr (Catalog #AAA330141) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330141 ELISA Kit recognizes Rat VLDLR. It is sometimes possible for the material contained within the vial of "Very Low-Density Lipoprotein Receptor (VLDLR), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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