Standard Curve (Sample)
(Fig.1. Rat Xanthine dehydrogenase (XDH) Standard Curve.)
Rat Xanthine Dehydrogenase (XDH) ELISA Kit | XDH elisa kit
Rat Xanthine Dehydrogenase (XDH) ELISA Kit
Gene Names
Xdh; XOR
Reactivity
Rat
Synonyms
Xanthine Dehydrogenase (XDH); N/A; Rat Xanthine Dehydrogenase (XDH) ELISA Kit; XDH; XO; XOR; xanthene dehydrogenase; xanthine oxidase; xanthine oxidoreductase; XDH elisa kit
Reactivity
Rat
Specificity
Rat Xanthine dehydrogenase (XDH) ELISA Kit has high sensitivity and excellent specificity for detection of Rat XDH. No significant cross-reactivity or interference between Rat XDH and analogues was observed.
Sequence Length
1331
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Rat Xanthine dehydrogenase (XDH) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Rat Xanthine dehydrogenase (XDH) Standard Curve.)
Related Product Information for XDH elisa kit
Description: This Rat Xanthine dehydrogenase (XDH) ELISA Kit employs a two-site sandwich ELISA to quantitate XDH in samples. An antibody specific for XDH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any XDH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XDH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XDH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The enzyme is a homodimer. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. The three substrates of this enzyme are xanthine, NAD+, and H2O, whereas its three products are urate, NADH, and H+.This enzyme belongs to the family of oxidoreductases, to be specific, those acting on CH or CH2 group with NAD+ or NADP+ as acceptor.Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism.
Background: Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The enzyme is a homodimer. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. The three substrates of this enzyme are xanthine, NAD+, and H2O, whereas its three products are urate, NADH, and H+.This enzyme belongs to the family of oxidoreductases, to be specific, those acting on CH or CH2 group with NAD+ or NADP+ as acceptor.Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
xanthine dehydrogenase/oxidase
NCBI Official Synonym Full Names
xanthine dehydrogenase
NCBI Official Symbol
Xdh
NCBI Official Synonym Symbols
XOR
NCBI Protein Information
xanthine dehydrogenase/oxidase
UniProt Protein Name
Xanthine dehydrogenase/oxidase
UniProt Gene Name
Xdh
UniProt Synonym Gene Names
XD; XO; XOR
UniProt Entry Name
XDH_RAT
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Product Notes
The Rat XDH xdh (Catalog #AAA330136) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330136 ELISA Kit recognizes Rat XDH. It is sometimes possible for the material contained within the vial of "Xanthine Dehydrogenase (XDH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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