SDS-PAGE
Carbonic Anhydrase II (CA2) Active Protein | CA2 active protein
Active Carbonic Anhydrase II (CA2)
Gene Names
Car2; Ca2
Reactivity
Rat
Applications
Activity Assay, Cell Culture
Purity
> 97%
Synonyms
Carbonic Anhydrase II (CA2); N/A; Active Carbonic Anhydrase II (CA2); CA-II; CAII; Car2; CAC; Carbonate dehydratase II; Carbonic anhydrase C; CA2 active protein
Host
Rat (Erythrocytes)
Reactivity
Rat
Purity/Purification
> 97%
Form/Format
Freeze-dried powder
Sequence Length
260
Applicable Applications for CA2 active protein
Activity Assay, Cell Culture
Tag
N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Isoelectric Point
6.9
SDS-PAGE
SDS-PAGE
Activity
(CA2 (Carbonic anhydrase 2) is an enzyme that catalyzes reversible hydration of carbon dioxide. It is essential for bone resorption and osteoclast differentiation and contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. It is widely accepted that CA2 also catalyzes hydrolysis of p-Nitrophenyl Acetate. Thus, a hydration assay was conducted to test the catalytic activity of CA2 using 4-Nitrophenyl Acetate (4-NPA) as substrate. Briefly, different concentrations of CA2 were incubated with 1mM 4-NPA in reaction buffer. The absorbance at the wavelength of 400nm was read per hour, and the result was shown in figure 1. It is obvious that CA2 catalyzes hydrolysis of p-Nitrophenyl Acetate.)
Activity
(CA2 (Carbonic anhydrase 2) is an enzyme that catalyzes reversible hydration of carbon dioxide. It is essential for bone resorption and osteoclast differentiation and contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. It is widely accepted that CA2 also catalyzes hydrolysis of p-Nitrophenyl Acetate. Thus, a hydration assay was conducted to test the catalytic activity of CA2 using 4-Nitrophenyl Acetate (4-NPA) as substrate. Briefly, different concentrations of CA2 were incubated with 1mM 4-NPA in reaction buffer. The absorbance at the wavelength of 400nm was read per hour, and the result was shown in figure 1. It is obvious that CA2 catalyzes hydrolysis of p-Nitrophenyl Acetate.)
Product Categories/Family for CA2 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Weight: 29kDa
Accurate Molecular Weight: 29kDa
Accurate Molecular Weight: 29kDa
NCBI Official Full Name
carbonic anhydrase 2
NCBI Official Synonym Full Names
carbonic anhydrase 2
NCBI Official Symbol
Car2
NCBI Official Synonym Symbols
Ca2
NCBI Protein Information
carbonic anhydrase 2
UniProt Protein Name
Carbonic anhydrase 2
UniProt Gene Name
Ca2
UniProt Synonym Gene Names
CA-II
Similar Products
Product Notes
The CA2 ca2 (Catalog #AAA148249) is an Active Protein produced from Rat (Erythrocytes) and is intended for research purposes only. The product is available for immediate purchase. The Active Carbonic Anhydrase II (CA2) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Carbonic Anhydrase II (CA2) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the CA2 ca2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase II (CA2), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.