FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-CD205 antibody NLDC145 (AAA72259). Paraformaldehyde fixed mouse thymocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of NLDC145 (AAA72259-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Rat anti-Mouse CD205 Antibody | anti-CD205 antibody
Anti-CD205 [NLDC145], Rat IgG2a
Gene Names
Ly75; CD205; DEC205; DEC-205
Reactivity
Mouse
Applications
Immunoblot, Western Blot, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Purity
Protein A affinity purified
Synonyms
CD205; N/A; Anti-CD205 [NLDC145], Rat IgG2a; Anti-CD205 [NLDC145]; Ly-75; NLDC-145; DEC205; DEC-205; Lymphocyte antigen 75; anti-CD205 antibody
Host
Rat
Reactivity
Mouse
Isotype
Rat IgG2a, Kappa
Clone Number
NLDC145
Specificity
This antibody is specific for murine DEC-205, a lectin endocytic receptor expressed at high levels by many dendritic cell subsets, where it mediates antigen uptake. The epitope recognised by this antibody is trypsin-sensitive.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD205 antibody
IB (Immunoblot), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry)
Immunogen
This antibody was raised by immunising Wistar rats with mouse lymph node stroma.
Origin Pub PMID
15829289
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-CD205 antibody NLDC145 (AAA72259). Paraformaldehyde fixed mouse thymocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of NLDC145 (AAA72259-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-CD205 antibody NLDC145 (AAA72259). Paraformaldehyde fixed mouse thymocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of NLDC145 (AAA72259-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence)
(Immunofluorescence staining of mouse thymocytes with anti-CD205 (AAA72259) NLDC145 Immunofluorescence analysis of paraformaldehyde fixed mouse thymocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of NLDC145 (AAA72259-23.0) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72259-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of mouse thymocytes with anti-CD205 (AAA72259) NLDC145 Immunofluorescence analysis of paraformaldehyde fixed mouse thymocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of NLDC145 (AAA72259-23.0) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72259-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-CD205 antibody
This antibody has been used in IHC analysis of acetone-fixed mouse lymph node sections and cytospin preparations (Krall et al, 1986), and immunofluorescence analysis of epidermal sheets (Krall et al, 1986).It has also been used in immunoblot experiments (Inaba et al, 1995) and in FC experiments to identify DEC-205-expressing cell subsets (Inaba et al, 1995; Hawiger et al, 2001).
Product Categories/Family for anti-CD205 antibody
References
Kraal et al. Langerhans' cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody J Exp Med. 1986 Apr 1; 163(4): 981-997 PMID:3950549
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
197,352 Da
NCBI Official Full Name
lymphocyte antigen 75
NCBI Official Synonym Full Names
lymphocyte antigen 75
NCBI Official Symbol
Ly75
NCBI Official Synonym Symbols
CD205; DEC205; DEC-205
NCBI Protein Information
lymphocyte antigen 75; ly-75
UniProt Protein Name
Lymphocyte antigen 75
UniProt Gene Name
Ly75
UniProt Synonym Gene Names
Cd205; Ly-75
UniProt Entry Name
LY75_MOUSE
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Product Notes
The CD205 ly75 (Catalog #AAA72259) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD205 [NLDC145], Rat IgG2a reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD205 can be used in a range of immunoassay formats including, but not limited to, IB (Immunoblot), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CD205 ly75 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD205, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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