Standard Curve (Sample)
Rat Neutrophil Gelatinase Associated Lipocalin (NGAL) RTU ELISA Kit | NGAL rtu elisa kit
Rat Neutrophil Gelatinase Associated Lipocalin (NGAL) ELISA Kit
Gene Names
Lcn2; Sip24
Reactivity
Rat
Synonyms
Neutrophil Gelatinase Associated Lipocalin (NGAL); N/A; Rat Neutrophil Gelatinase Associated Lipocalin (NGAL) ELISA Kit; LCN2; p25; Lipocalin 2; Oncogene 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Siderocalin LCN2; NGAL rtu elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of NGAL. No significant cross-reactivity or interference between NGAL and analogues was observed.
Sequence Length
198
Samples
Rat Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates Or Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
78.1-5000pg/mL
Sensitivity
27pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NGAL were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NGAL were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for NGAL rtu elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of NGAL in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NGAL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NGAL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NGAL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NGAL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NGAL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NGAL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NGAL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NGAL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,476 Da
NCBI Official Full Name
neutrophil gelatinase-associated lipocalin
NCBI Official Synonym Full Names
lipocalin 2
NCBI Official Symbol
Lcn2
NCBI Official Synonym Symbols
Sip24
NCBI Protein Information
neutrophil gelatinase-associated lipocalin
UniProt Protein Name
Neutrophil gelatinase-associated lipocalin
UniProt Gene Name
Lcn2
UniProt Synonym Gene Names
NGAL
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Product Notes
The Rat NGAL lcn2 (Catalog #AAA219610) is a RTU ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA219610 RTU ELISA Kit recognizes Rat NGAL. It is sometimes possible for the material contained within the vial of "Neutrophil Gelatinase Associated Lipocalin (NGAL), RTU ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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