Application Data
(Fig. 2: A431 cells were detached with Celltase @ RT with 5 and 15 minutes of incubation time. Cells were washed, harvested and stained using anti-Epcam antibody . Anti mouse PE was used as secondary antibody. Cells were analyzed by flow cytometry and both the files were overlapped. Expression of Epcam antigen on the cell surface after Celltase treatment was similar in both the time points. Celltase is gentle on surface antigens and cell membrane unlike Trypsin.)
Celltase Cell Detachment Solution Reagent
Celltase Cell Detachment Solution
Synonyms
Celltase Cell Detachment Solution; N/A; Celltase Cell Detachment Solution reagent
Form/Format
1X Celltase enzyme solution containing aqueous buffer, EDTA and Phenol Red. Sterile-filtered, suitable for cell culture.
Preparation and Storage
Store for 2 months at 2-8 degree C. 12 months if frozen at -20 degree C.
For long term or intermittent use the product may be aliquoted and frozen if necessary.
For long term or intermittent use the product may be aliquoted and frozen if necessary.
Application Data
(Fig. 2: A431 cells were detached with Celltase @ RT with 5 and 15 minutes of incubation time. Cells were washed, harvested and stained using anti-Epcam antibody . Anti mouse PE was used as secondary antibody. Cells were analyzed by flow cytometry and both the files were overlapped. Expression of Epcam antigen on the cell surface after Celltase treatment was similar in both the time points. Celltase is gentle on surface antigens and cell membrane unlike Trypsin.)
Application Data
(Fig. 2: A431 cells were detached with Celltase @ RT with 5 and 15 minutes of incubation time. Cells were washed, harvested and stained using anti-Epcam antibody . Anti mouse PE was used as secondary antibody. Cells were analyzed by flow cytometry and both the files were overlapped. Expression of Epcam antigen on the cell surface after Celltase treatment was similar in both the time points. Celltase is gentle on surface antigens and cell membrane unlike Trypsin.)
Application Data
(Fig. 1: Human A431 epidermoid carcinoma cells cultured in DMEM + 10% FBS were treated with Celltase at room temperature for different time points. Treatment resulted in rapid cell detachment and high viability. Cell viability was 96+/-4% even after 60 minutes of Celltase treatment.)
Application Data
(Fig. 1: Human A431 epidermoid carcinoma cells cultured in DMEM + 10% FBS were treated with Celltase at room temperature for different time points. Treatment resulted in rapid cell detachment and high viability. Cell viability was 96+/-4% even after 60 minutes of Celltase treatment.)
Related Product Information for Celltase Cell Detachment Solution reagent
Celltase is a ready-to-use cell detachment solution of made up of proteolytic and collagenolytic enzymes. Celltase is a direct replacement for Trypsin solution and is very useful for the routine cell disassociation from standard tissue culture plastic-ware and other adhesion coated polymers. Celltase is devoid of any mammalian and bacterial derived products. It performs efficiently in detaching cells for the analysis of cell surface markers, routine cell passage, proliferation assays, production scale-up of bioreactors, various bioassays and flow cytometry among others. Primary cells tested for Celltase application include fibroblasts, keratinocytes, hepatocytes, mouse bone marrow cells, hESCs, macrophages and PBMCs, neuronal stem cells. Celltase is shown to be effective on A431, HeLa, 293 cells, L929 cells, 3T3, A549, adherent CHO and BHK cells, HT1080 fibrosarcoma cells, Sf9 insect cells, Vero, COS, NT2, MG63, M24. As compared to Trypsin, CelltaseTM is gentle on cells and it auto-inhibits at 37 degree C.
Product Categories/Family for Celltase Cell Detachment Solution reagent
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Product Notes
The Celltase Cell Detachment Solution (Catalog #AAA78394) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Celltase Cell Detachment Solution, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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