Application Data
(Figure 2. Accelerated stability test of AAA129417. Following lyophilization, samples were stored at -20°C (black), 37°C for 1 day (red), 37°C for 2 days (blue), and 37°C for 3 days (green), separately. Upon reconstitution, the cell inhibition rate of each sample was determined using the CCK8 method. The data indicate excellent stability for all samples.)
DiTag MMAE IgG Reagent | IgG reagent
DiTag MMAE IgG labeling reagent
Synonyms
DiTag MMAE IgG; N/A; DiTag MMAE IgG labeling reagent; IgG reagent
Isotype
The DiTag MMAE IgG labeling reagents can be used for human IgG1, IgG2 and IgG4, rabbit IgG, mouse IgG2a and IgG2b.
Form/Format
Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Detection Method
Cell viability detection with MTT, CCK8, or CTG
Preparation and Storage
The reagents are supplied in lyophilized form. We recommend storing the vial (s) at -20?, desiccated and protected from light. Once reconstituted, the reagents can be stored at 2-8? for 1~2 weeks, or with 50% glycerol at -20?.
Application Data
(Figure 2. Accelerated stability test of AAA129417. Following lyophilization, samples were stored at -20°C (black), 37°C for 1 day (red), 37°C for 2 days (blue), and 37°C for 3 days (green), separately. Upon reconstitution, the cell inhibition rate of each sample was determined using the CCK8 method. The data indicate excellent stability for all samples.)
Application Data
(Figure 2. Accelerated stability test of AAA129417. Following lyophilization, samples were stored at -20°C (black), 37°C for 1 day (red), 37°C for 2 days (blue), and 37°C for 3 days (green), separately. Upon reconstitution, the cell inhibition rate of each sample was determined using the CCK8 method. The data indicate excellent stability for all samples.)
Application Data
(Figure 1. Cell inhibition rate of Hela detected by CCK8 method. The IC50 of B7H3 ADC BMK is 66ng/ml, indicating specific internalization.)
Application Data
(Figure 1. Cell inhibition rate of Hela detected by CCK8 method. The IC50 of B7H3 ADC BMK is 66ng/ml, indicating specific internalization.)
Related Product Information for IgG reagent
DiTag MMAE IgG labeling reagents provide an easy solution for quantifying antibody internalization activities. Leveraging VcMMAE (MC-VC-PABC-MMAE) conjugated to an Fc binding protein, these reagents bind to IgG antibodies from various species, resulting in the formation of a VcMMAE-labeled antibody-reagent complex. Upon antibody internalization, the cleavable linker MC-VC-PABC is enzymatically cleaved by cathepsin B, a protein overexpressed in multiple cancer types. This enzymatic cleavage triggers the release of PABC-substituted MMAE, forming an unstable intermediate that liberates the free drug. Measurement of cell killing or inhibition allows researchers to evaluate the efficiency of antibody internalization into cells. This critical information enhances our understanding of the cellular uptake mechanism of antibodies and aids in assessing their efficacy in targeted therapies or test applications.
Product Categories/Family for IgG reagent
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Product Notes
The IgG (Catalog #AAA129417) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "DiTag MMAE IgG, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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