MTT reagent
MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) Solution
Synonyms
MTT; N/A; MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) Solution; 5mg/mL MTT solution; MTT reagent
Form/Format
Liquid; 10mM PBS.
Concentration
5mg/mL (varies by lot)
pH
7.2-7.4
Notes
1. This product is for research use only.
2. This product is sterilized by 0.22 um filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the pollution.
4. The MTT solution decomposes easily in the light. Please avoid light during operation. Do not use MTT solution when it turns grey-green.
5. MTT solution will solidify at low temperature, Please incubate the solution at 20-25 degree C water bath and use it when the solution is thoroughly dissolved.
6. Please mix MTT solution fully before use and do not store it at room temperature for a long time. It can be stored at 2-8 degree C with shading light for a month.
7. Avoid repeated freeze-thaw cycles. It is recommended to aliquot the solution into smaller quantities for optimal storage.
8. MTT is carcinogenic, please take safety precautions and follow the procedures of laboratory reagent operation. All waste liquid should be handled in accordance with local regulations.
2. This product is sterilized by 0.22 um filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the pollution.
4. The MTT solution decomposes easily in the light. Please avoid light during operation. Do not use MTT solution when it turns grey-green.
5. MTT solution will solidify at low temperature, Please incubate the solution at 20-25 degree C water bath and use it when the solution is thoroughly dissolved.
6. Please mix MTT solution fully before use and do not store it at room temperature for a long time. It can be stored at 2-8 degree C with shading light for a month.
7. Avoid repeated freeze-thaw cycles. It is recommended to aliquot the solution into smaller quantities for optimal storage.
8. MTT is carcinogenic, please take safety precautions and follow the procedures of laboratory reagent operation. All waste liquid should be handled in accordance with local regulations.
Preparation and Storage
Store at -20°C, shading light
Shipping: Ships on Dry Ice.
Shipping: Ships on Dry Ice.
Related Product Information for MTT reagent
MTT(3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) is widely used for determination of cell proliferation and cytotoxicity. The MTT can be reduced by succinate dehydrogenase in mitochondria of living cells to form crystalline dark purple product formazan.Formazan was deposited in cells, but dead cells do not. The formazan can be solubilized in dimethyl sulfoxide (DMSO).The absorbance (OD value) was measured at the wavelength of 490nm or 570nm. The number or activity of living cells could be determined by the OD value. Within a certain range of cell numbers, the amount of MTT crystal formation was proportional to the number or activity of living cells.
Product Categories/Family for MTT reagent
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Product Notes
The MTT (Catalog #AAA22222) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "MTT, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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