Application Data
(Actin filaments (green) were stained with Phalloidin - AF488)
Phalloidin Reagent
Phalloidin - AF488
Applications
Immunofluorescence
Synonyms
Phalloidin; N/A; Phalloidin - AF488; Phalloidin reagent
Form/Format
This product is lyophilized powder, and it is difficult to observe the trace amount. Please centrifuge before use, and add appropriate solvent to dissolve it. The solution after dissolution is almost transparent.
Applicable Applications for Phalloidin reagent
IF (Immunofluorescence)
Samples
formaldehyde-fixed, permeabilized tissue sections, cell cultures or cellfree experiments
Conjugation
AF488
Directions for Use
Staining fixed cellsThe following protocol describes the staining procedure for adherent cells grown on glass coverslips or 8-well chamber slides. Phalloidins also can be used to stain fixed frozen or paraffin tissue sections, as well as yeast and fungi. 1. Wash cells 3 times with PBS. 2. Fix cells on ice with 4% formaldehyde solution in PBS for 15 minutes. Note: Methanol can disrupt actin during the fixation process. Therefore, it is best to avoid any methanol containing fixatives or other solvent-based fixatives. The preferred fixative is methanol-free formaldehyde. 3. Wash cells 3 times with PBS. 4. Permeabilize cells with 0.5% Triton X-100 in PBS at room temperature for 10 minutes. 5. Wash cells 3 times with PBS. 6. Dilute 1-5 ul fluorescent phalloidin stock solution in 200 ul PBS for each cover slip or chamber to be stained. Place the staining solution on the coverslip for 20 minutes at room temperature.Note?Staining volume can be adjusted according to the sample. To avoid evaporation, keep the coverslips inside a covered container and the chamber slides covered during the incubation. 7. Wash 2-3 times with PBS. 8. Image using fluorescence microscopy. Fluorescent phalloidins are photostable enough to image in PBS, but for best results we recommend mounting with antifade mounting medium.Staining living cellsFluorescently-labeled phalloidin is not cell-permeant and has therefore has not been used extensively with living cells. However, living cells have been labeled by pinocytosis or unknown mechanism. In general, a larger amount of stain will be needed for staining living cells. Alternatively, ?uorescent phalloidins have also been injected into cells for monitoring actin distribution and cell motility.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C and protect from light for 12 months.
Application Data
(Actin filaments (green) were stained with Phalloidin - AF488)
Application Data
(Actin filaments (green) were stained with Phalloidin - AF488)
Application Data
(Actin filaments (green) were stained with Phalloidin - AF488)
Application Data
(Actin filaments (green) were stained with Phalloidin - AF488)
Related Product Information for Phalloidin reagent
AF488 labled Phalloidin bind to F-actin
Product Categories/Family for Phalloidin reagent
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Product Notes
The Phalloidin (Catalog #AAA266233) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Phalloidin can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence). Researchers should empirically determine the suitability of the Phalloidin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phalloidin, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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