Staurosporine Reagent
Staurosporine
Purity
>98%
Synonyms
Staurosporine; N/A; Staurosporine reagent
Purity/Purification
>98%
Formula
C28H26N4O3
CAS Number
62996-74-1
Solubility
DMSO (50 mM)
Ethanol (2 mg/mL)
Methanol (2 mg/mL)
Insoluble in water
Ethanol (2 mg/mL)
Methanol (2 mg/mL)
Insoluble in water
How To Use
1. Dissolve staurosporine powder in tissue culture grade DMSO to obtain a 1 mM staurosporine stock concentration.
Example Calculation:
Staurosporine Formula Weight = 466.5
1 mM Staurosporine = 1 x 466.5 ug/mL = 0.4665 mg/mL
To reconstitute a 1 mg vial of Staurosporine in DMSO:
1 mg/0.4665 mg/mL = 2.14 mL DMSO
2. Prepare 50-100 uL aliquots of the DMSO solublilized stauro-sporine and store frozen at -20°C. A frozen vial of staurospo-rine may be thawed twice before discarding.
3. Spike cell cultures at a staurosporine concentration of 1 uM in cell culture media. This equates to a 1 uL spike of the 1 mM staurosporine stock per mL of the cell culture suspension. This concentration works well for inducing many cell lines, including HL-60 and Jurkat cells when using a 4-5 hour incu-bation period at 37°C. Typical cell suspension concentrations that have been used for this staurosporine induction protocol range from 1 x 105-1 x 106 cells/mL.
4. Perform time course studies on your particular cell line to determine the optimal staurosporine concentration and exposure time required to achieve good apoptosis induction levels.
5. Proceed with your experimental apoptosis induction model system.
Example Calculation:
Staurosporine Formula Weight = 466.5
1 mM Staurosporine = 1 x 466.5 ug/mL = 0.4665 mg/mL
To reconstitute a 1 mg vial of Staurosporine in DMSO:
1 mg/0.4665 mg/mL = 2.14 mL DMSO
2. Prepare 50-100 uL aliquots of the DMSO solublilized stauro-sporine and store frozen at -20°C. A frozen vial of staurospo-rine may be thawed twice before discarding.
3. Spike cell cultures at a staurosporine concentration of 1 uM in cell culture media. This equates to a 1 uL spike of the 1 mM staurosporine stock per mL of the cell culture suspension. This concentration works well for inducing many cell lines, including HL-60 and Jurkat cells when using a 4-5 hour incu-bation period at 37°C. Typical cell suspension concentrations that have been used for this staurosporine induction protocol range from 1 x 105-1 x 106 cells/mL.
4. Perform time course studies on your particular cell line to determine the optimal staurosporine concentration and exposure time required to achieve good apoptosis induction levels.
5. Proceed with your experimental apoptosis induction model system.
Preparation and Storage
Store at -20°C. Avoid freeze/thaw cycles.
Related Product Information for Staurosporine reagent
Staurosporine is a natural product derived alkaloid which is isolated from Streptomyces staurospores cultures. This protein kinase inhibitor belongs to a family of kinase inhibitors containing an indole carbozol chromophore. Staurosporine is a potent inhibitor of a number of kinases including: protein kinase C (PKC), cAMP dependent protein kinase, protein kinase A (PKA), tyrosine protein kinase, phosphorylase kinase, and Ca2+/calmodulan-dependent protein kinase. The ATP binding site on these kinase enzymes appears to be targeted by these Streptomyces derived inhibitors. Experiments using [3H]- staurosporine inhibition of purified PKC, PKA, tyrosine protein kinase, and Ca2+/calmodulan-dependent protein kinase enzymes revealed dissociation constants (KD) ranging from 2-10 nM. Human promyelocytic leukemia (HL-60) cells were found to be sensitive to apoptosis induction from a 2 hour exposure to staurosporine concentrations as low as 0.1 uM.
Inhibition of these intracellular kinases by staurosporine leads to the induction of apoptosis as exhibited by the classic chromatin condensation leading to the formation of micronuclear bodies and reduced cell volumes. DNA is subsequently cleaved into oligonucleosomal fragments (laddering) as evidenced by agarose gel analysis. Staurosporine was determined to be an effective apoptosis-inducing agent in virtually all cell lines that were exposed to this kinase inhibiting agent.
Inhibition of these intracellular kinases by staurosporine leads to the induction of apoptosis as exhibited by the classic chromatin condensation leading to the formation of micronuclear bodies and reduced cell volumes. DNA is subsequently cleaved into oligonucleosomal fragments (laddering) as evidenced by agarose gel analysis. Staurosporine was determined to be an effective apoptosis-inducing agent in virtually all cell lines that were exposed to this kinase inhibiting agent.
Product Categories/Family for Staurosporine reagent
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Product Notes
The Staurosporine (Catalog #AAA52741) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Staurosporine, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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