Taq 2X MeanGreen Master Mix Reagent
Taq 2X MeanGreen Master Mix
Synonyms
Taq 2X MeanGreen Master Mix; N/A; Taq 2X MeanGreen Master Mix reagent
Concentration
2X (varies by lot)
Protocol
The following reaction set up and general cycling conditions are recommended but can vary depending on the template and primers being used:
Component: Taq 2X MaxGreen MasterMix , Volume: 25ul , Final Concentration:1X ;
Component: Upstream Primer, 10uM , Volume: 0.5-5.0 ul , Final Concentration: 0.1-1.0um ;
Component: Downstream Primer, 10um ; Volume: 0.5-5.0 ul, Final Concentration: 0.1-1.0um ;
Component: DNA Template , Volume: Xul , Final Concentration: >1ng ;
Compound: Nuclease Free Water to Volume, Volume: 50ul , Final Concentration: N/A
Component: Taq 2X MaxGreen MasterMix , Volume: 25ul , Final Concentration:1X ;
Component: Upstream Primer, 10uM , Volume: 0.5-5.0 ul , Final Concentration: 0.1-1.0um ;
Component: Downstream Primer, 10um ; Volume: 0.5-5.0 ul, Final Concentration: 0.1-1.0um ;
Component: DNA Template , Volume: Xul , Final Concentration: >1ng ;
Compound: Nuclease Free Water to Volume, Volume: 50ul , Final Concentration: N/A
Thermo Cycling Conditions
The following general cycling conditions are recommended but can vary depending on the template and primers being used:
Cycling Step: Initial Denaturation , Tempeture: 95°C, Holding Time: 2min, Cycles: 1
Cycling Step: Denaturation, Tempeture: 94-96°C, Holding Time: 15-30 sec, Cycles: 20-30
Cycling Step: Annealing#, Tempeture: 55-65°C, Holding Time:15-60 sec., Cycles: 20-30
Cycling Step: Extension, Temperature:70-72°C, Holding Time:1min/kb, Cycles: 20-30
Cycling Step: Final Extension, Temperature: 70-72°C, Holding Time: 0-10 min , Cycles: 1
#Annealing will depend on primer length and composition. Generally, begin 5°C below primer TM
Cycling Step: Initial Denaturation , Tempeture: 95°C, Holding Time: 2min, Cycles: 1
Cycling Step: Denaturation, Tempeture: 94-96°C, Holding Time: 15-30 sec, Cycles: 20-30
Cycling Step: Annealing#, Tempeture: 55-65°C, Holding Time:15-60 sec., Cycles: 20-30
Cycling Step: Extension, Temperature:70-72°C, Holding Time:1min/kb, Cycles: 20-30
Cycling Step: Final Extension, Temperature: 70-72°C, Holding Time: 0-10 min , Cycles: 1
#Annealing will depend on primer length and composition. Generally, begin 5°C below primer TM
Functionality
Taq 2x Mean GreenTM Master Mix is tested for performance in a polymerase chain reaction (PCR) using 50ul reaction volumes. Reactions containing the master mix, template DNA, and specific primers resulted in the expexted product from Human gDNA in comparison to a control lot of MasterMix.
Passing Result: Pass
Results: Pass
Passing Result: Pass
Results: Pass
Unspecific dsEndonucleases
Taq 2X MeansGreenTMMaster Mix is tested for nuclease degradationin reactions containing a dsDNA or dsDNA substrate.
Passing Result: Pass
Results: Pass
Passing Result: Pass
Results: Pass
E. coli Contamination
Taq 2X MeanGreenTM Master Mix is used as the template in a PCR reaction using primers specific to an E. coli gene No E.coli DNA is detected before 40 cycles.
Passing Result :Pass
Results: Pass
Passing Result :Pass
Results: Pass
HdDNA Contamination
Taq 2X MeanGreenTM Master Mix is used as the temeplate in a PCR reaction using primers specific to Human gsDNA. No Human gDNA is detected before 40 cycles.
Passing Result: Pass
Result: Pass
Passing Result: Pass
Result: Pass
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Upon receipt, store at -20°C. Room temperature for up to 60 days, 4°C for up to 3 months. Minimize freeze thaw of master mix to avoid loss of performance.
Related Product Information for Taq 2X MeanGreen Master Mix reagent
Taq 2X MeanGreen™ MasterMix:
Supplied as 2X reaction buffer with loading Dye dCTP, dGTP, dATP. dTTP. MgCI2 and Taq DNA polymerase Taq DNA Polymerase gene was isolated from Thermus aquaticus YT1 and expressed in E coli. Empirical's Taq 2X MeanGreenTM MasterMix is easy to use, versatile. and provides excellent amplification for many PCR based applications. Just add template and primers with the MeanGreenTM MasterMix and the reaction is ready to go.
Taq 2X MeanGreenTM MasterMix contains blue and yellow loading dyes and a density agent that allow reactions to be loaded directly onto agarose gels and make monitoring progress during electrophoresis easy. These dyes do not obscure visualization of DNA bands on the gel as the dye fronts run outside the size of most PCR products.
Supplied as 2X reaction buffer with loading Dye dCTP, dGTP, dATP. dTTP. MgCI2 and Taq DNA polymerase Taq DNA Polymerase gene was isolated from Thermus aquaticus YT1 and expressed in E coli. Empirical's Taq 2X MeanGreenTM MasterMix is easy to use, versatile. and provides excellent amplification for many PCR based applications. Just add template and primers with the MeanGreenTM MasterMix and the reaction is ready to go.
Taq 2X MeanGreenTM MasterMix contains blue and yellow loading dyes and a density agent that allow reactions to be loaded directly onto agarose gels and make monitoring progress during electrophoresis easy. These dyes do not obscure visualization of DNA bands on the gel as the dye fronts run outside the size of most PCR products.
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Product Notes
The Taq 2X MeanGreen Master Mix (Catalog #AAA57605) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Taq 2X MeanGreen Master Mix, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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