Application Data
(Fig. The sample is Mouse TNF-alpha protein , the primary antibody is TNF-alpha Polyclonal Antibody , and the secondary antibody is HRP, Goat Anti-Rabbit IgG . The exposure time is 30s.)
West Pico PLUS Chemiluminescent Substrate Reagent
West Pico PLUS Chemiluminescent Substrate
Applications
Immunoprecipitation, Western Blot
Synonyms
West Pico PLUS Chemiluminescent Substrate; N/A; Enhanced Chemiluminescence; ECL; Chemiluminescence; Electrochemiluminescence; West Pico PLUS Chemiluminescent Substrate reagent
Form/Format
Liquid, ready to use.
Applicable Applications for West Pico PLUS Chemiluminescent Substrate reagent
IP (Immunoprecipitation), WB (Western Blot)
Product Application
Advantages: High sensitivity
Mechanism: Add unique immune signal enhancement components
Application: Easier to obtain detection signals, better detection of low-abundance proteins (picogram level)
Advantages: Signal stability
Mechanism: The signal is strong and lasts for 4 hours
Application: The signal is more stable, the signal can still be obtained after 4h color development of the signal film
Advantages: Easy operation
Mechanism: Contains optimized antibody stabilization components
Application: Increase the storage time of the diluted antibody, and can be used repeatedly
Advantages: High compatibility
Mechanism: Suitable for PVDF membrane and NC membrane
Application: Good compatibility with a variety of membrane types, worry-free use
Advantages: Stable performance
Mechanism: Stored at 4 degree C for one year, there is no difference in color rendering effect
Application: Can be stored for more than one year without affection on use
Advantages: Universal detection
Mechanism: Can be detected with X-ray film and chemiluminescence imager
Application: Can get good results through a variety of instruments
Mechanism: Add unique immune signal enhancement components
Application: Easier to obtain detection signals, better detection of low-abundance proteins (picogram level)
Advantages: Signal stability
Mechanism: The signal is strong and lasts for 4 hours
Application: The signal is more stable, the signal can still be obtained after 4h color development of the signal film
Advantages: Easy operation
Mechanism: Contains optimized antibody stabilization components
Application: Increase the storage time of the diluted antibody, and can be used repeatedly
Advantages: High compatibility
Mechanism: Suitable for PVDF membrane and NC membrane
Application: Good compatibility with a variety of membrane types, worry-free use
Advantages: Stable performance
Mechanism: Stored at 4 degree C for one year, there is no difference in color rendering effect
Application: Can be stored for more than one year without affection on use
Advantages: Universal detection
Mechanism: Can be detected with X-ray film and chemiluminescence imager
Application: Can get good results through a variety of instruments
Application suggestion
1. Routine electrophoresis, membrane transfer, HRP-labeled antibody incubation, and membrane washing. It is recommended to use SuperKine™ Enhanced Antibody Dilution Buffer (MBS9719196) to dilute the antibody. Universal Loading Control Antibody Cocktail (MBS9719226) is used for the sample internal reference detection. It is recommended to use HRP-labeled IgG, such as, HRP goat anti-rabbit secondary antibody (MBS9718740).
2. The diluted antibody should be stored at 4°C immediately after the antibody incubation, so that it can be reused later. While washing the HRP-labeled secondary antibody on the membrane, freshly prepare the luminescence working solution, mixing the two reagents at 1:1 ratio to prepare the working solution.
3. If the size of the blotting membrane is 1 cm2, it is recommended to use 0.1-0.2 ml of SuperKine™ West Pico PLUS Chemiluminescent Substrate working solution.
4. Incubate in the ECL working solution for 1-5 minutes.
5. Clamp the membrane with tweezers, and gently touch the lower edge of the membrane with the filter paper to remove excess luminescent liquid on the membrane. Cover the blotting membrane with a transparent plastic wrap.
6. Expose to X-ray film or take photo by chemiluminescence imager.
2. The diluted antibody should be stored at 4°C immediately after the antibody incubation, so that it can be reused later. While washing the HRP-labeled secondary antibody on the membrane, freshly prepare the luminescence working solution, mixing the two reagents at 1:1 ratio to prepare the working solution.
3. If the size of the blotting membrane is 1 cm2, it is recommended to use 0.1-0.2 ml of SuperKine™ West Pico PLUS Chemiluminescent Substrate working solution.
4. Incubate in the ECL working solution for 1-5 minutes.
5. Clamp the membrane with tweezers, and gently touch the lower edge of the membrane with the filter paper to remove excess luminescent liquid on the membrane. Cover the blotting membrane with a transparent plastic wrap.
6. Expose to X-ray film or take photo by chemiluminescence imager.
Highlight moment
Except the enhanced ECL luminescent solution, there are many ways to improve the signal for immunological experiments, such as using SuperKine™ Enhanced Antibody Dilution Buffer (MBS9719196) in WB experiment, choosing DyLight, IFKine™ specialized secondary antibodies in IF experiment, or adopting high-quality animal serum, these are all pretty good options.
Precautions
1. Do not mix components from different batch numbers and different manufacturers; otherwise, it may cause abnormal results.
2. In order to obtain the best experiment results, you need to optimize all of your experiment elements, including the number of samples, antibody concentration, as well as the use of membranes and blocking reagents.
3. Mix the two substrate components at 1:1 ratio to prepare a substrate working solution. Please pay attention to change the tips during the aspiration process of A and B solution.
4. SuperKine™ West Pico PLUS Chemiluminescent Substrate has a long luminescent duration, but it is best to perform compression or imaging within 30 minutes of color development.
5. It is recommended that every 1 cm2 membrane corresponds to 0.1-0.2 mL SuperKine™ West Pico PLUS Chemiluminescent Substrate working solution (picogram level).
2. In order to obtain the best experiment results, you need to optimize all of your experiment elements, including the number of samples, antibody concentration, as well as the use of membranes and blocking reagents.
3. Mix the two substrate components at 1:1 ratio to prepare a substrate working solution. Please pay attention to change the tips during the aspiration process of A and B solution.
4. SuperKine™ West Pico PLUS Chemiluminescent Substrate has a long luminescent duration, but it is best to perform compression or imaging within 30 minutes of color development.
5. It is recommended that every 1 cm2 membrane corresponds to 0.1-0.2 mL SuperKine™ West Pico PLUS Chemiluminescent Substrate working solution (picogram level).
Preparation and Storage
Protected from light, store at 4°C, stable for 12 months from date of shipment
Application Data
(Fig. The sample is Mouse TNF-alpha protein , the primary antibody is TNF-alpha Polyclonal Antibody , and the secondary antibody is HRP, Goat Anti-Rabbit IgG . The exposure time is 30s.)
Application Data
(Fig. The sample is Mouse TNF-alpha protein , the primary antibody is TNF-alpha Polyclonal Antibody , and the secondary antibody is HRP, Goat Anti-Rabbit IgG . The exposure time is 30s.)
Related Product Information for West Pico PLUS Chemiluminescent Substrate reagent
Principle of the Assay: After years of development on chemiluminescence technology, there have been a variety of luminescence systems, but the most commonly used one in laboratories is still a technology system based on Luminol or its derivatives (isoluminol, etc.). Western luminescent detection reagent ECL immunoblotting chemiluminescence solution is a non-radioactive (horseradish peroxidase) luminescence system designed to detect trace protein immobilized on a solid membrane (such as NC, PVDF, etc.). It is an experimental auxiliary reagent for the photosensitive recording of its immunoblotting by X-ray film (radiograph).
Product Categories/Family for West Pico PLUS Chemiluminescent Substrate reagent
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Product Notes
The West Pico PLUS Chemiluminescent Substrate (Catalog #AAA332506) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's West Pico PLUS Chemiluminescent Substrate can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the West Pico PLUS Chemiluminescent Substrate for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "West Pico PLUS Chemiluminescent Substrate, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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