NF-kappaB p65 ELISA Kit | RELA elisa kit

NF-kappaB p65 (Phospho-Ser276) Phospho Sandwich ELISA Kit

Cat. Num. AAA318626

• Gene Names: RELA; p65; CMCU; NFKB3
• Reactivity: Human, Mouse, Rat
• Synonyms: NF-kappaB p65; N/A; NF-kappaB p65 (Phospho-Ser276) Phospho Sandwich ELISA Kit; NFKB3; nuclear factor NF-kappa-B p65 subunit; p65; RELA; TF65; transcription factor p65; RELA elisa kit

• Reactivity: Human, Mouse, Rat
• Specificity: The NF-kappaB p65 (Phospho-Ser276) Phospho Sandwich ELISA Kit is a convenient, lysate- free, high throughput and sensitive assay kit that can detect NF-kappaB p65 protein phosphorylation and expression profile in cell lysates. The kit can be used for measuring the relative amounts of phosphorylated NF-kappaB p65 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on NF-kappaB p65 phosphorylation.
• Sequence Length: 548
• Assay Type: Quantitative Sandwich

• Preparation and Storage: Store entire kit at 4 degree C until use. Kit expiration is 3 months from date of shipment.

ELISA

(Enzyme-Linked Immunosorbent Assay (ELISA) for immunogen phosphor-peptide (left) and non-phospho peptide (right), using Anti-NF-kB p65 (Phospho-Ser276) Antibody.)

Application Data

(Jurkat cells were grown to 90% confluency and were stimulated with U.V. 150 J/s and incubated for 1 hr. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of NF-kB p65 P-Ser276 versus untreated Jurkat lysates.)

Related Product Information for RELA elisa kit

Principle of the Assay: The Phospho Sandwich NF-kappaB p65 (Phospho-Ser276) ELISA Kit contains the components necessary for semi-quantitative determination of NF-kappaB p65 (Phospho-Ser276) concentrations within experimental cell lysate samples. This particular immunoassay utilizes the traditional "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope NF-kappaB p65 (Phospho-Ser276) while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer where data analysis may begin.

Product Categories/Family for RELA elisa kit

Phospho Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 223468681
• NCBI GeneID: 5970
• NCBI Accession #: NP_001138610.1
• NCBI GenBank Nucleotide #: NP_001138610.1
• UniProt Accession #: Q04206
• NCBI Official Full Name: transcription factor p65 isoform 2
• NCBI Official Synonym Full Names: RELA proto-oncogene, NF-kB subunit
• NCBI Official Symbol: RELA
• NCBI Official Synonym Symbols: p65; CMCU; NFKB3
• NCBI Protein Information: transcription factor p65
• UniProt Protein Name: Transcription factor p65
• UniProt Gene Name: RELA
• UniProt Synonym Gene Names: NFKB3
• UniProt Entry Name: TF65_HUMAN