Mouse v-rel reticuloendotheliosis viral oncogene homolog A (avian) ELISA Kit | RELA elisa kit

Mouse nuclear factor- degree B p65, NF- degree B p65 ELISA Kit

Cat. Num. AAA79632

• Gene Names: Rela; p65
• Reactivity: Mouse
• Synonyms: v-rel reticuloendotheliosis viral oncogene homolog A (avian); N/A; Mouse nuclear factor- degree B p65, NF- degree B p65 ELISA Kit; Mouse nuclear factor-kappaB p65; NF-kappaB p65 ELISA Kit; MGC131774; NFKB3; p65; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene; RELA elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse NF-kB p65. No significant cross-reactivity or interference between mouse NF-kB p65 and analogues was observed.
• Sequence Length: 549
• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 31.25 pg/ml -2000 pg/ml.
• Sensitivity: The minimum detectable dose of mouse NF-kB p65 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for RELA elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NF-kappaB p65 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NF-kappaB p65 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NF-kappaB p65 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NF-kappaB p65 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6677709
• NCBI GeneID: 19697
• NCBI Accession #: NP_033071.1
• NCBI GenBank Nucleotide #: NM_009045.4
• UniProt Accession #: Q04207; Q62025
• Molecular Weight: 60,212 Da
• NCBI Official Full Name: transcription factor p65
• NCBI Official Synonym Full Names: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• NCBI Official Symbol: Rela
• NCBI Official Synonym Symbols: p65
• NCBI Protein Information: transcription factor p65; p65 NFkB; p65 NF kappaB; p65 NF-kappa B; nuclear factor NF-kappa-B p65 subunit; avian reticuloendotheliosis viral (v-rel) oncogene homolog A; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
• UniProt Protein Name: Transcription factor p65
• UniProt Gene Name: Rela
• UniProt Synonym Gene Names: Nfkb3
• UniProt Entry Name: TF65_MOUSE