Human Resistin CLIA Kit | RETN clia kit

Resistin (Human) LumiAb ELISA Kit

Cat. Num. AAA318558

• Gene Names: RETN; ADSF; RSTN; XCP1; FIZZ3; RETN1
• Reactivity: Human
• Synonyms: Resistin; N/A; Resistin (Human) LumiAb ELISA Kit; Human Resistin; Adipose tissue-specific secretory factor; ADSF; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; RETN clia kit

• Reactivity: Human
• Specificity: The LumiAb Human Pentraxin-3 ELISA is capable of recognizing both recombinant and naturally produced Human Pentraxin-3 proteins. The antigens listed below were tested at 100 ng/ml and did not exhibit significant cross reactivity or interference.
• Sequence Length: 108
• Assay Type: Quantitative Sandwich
• Sensitivity: 73 pg/ml.

• Preparation and Storage: Store at 4 degree C for 6 months.

Standard Curve (Sample)

Related Product Information for RETN clia kit

Principle of the Assay: The LumiAb Human Pentraxin-3 Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human Pentraxin-3 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human Pentraxin-3 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Pentraxin 3/TSG-14 plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, and possibly clearance of self-components and female fertility.

Product Categories/Family for RETN clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 12584202
• NCBI GeneID: 56729
• UniProt Accession #: Q9HD89
• NCBI Official Full Name: resistin
• NCBI Official Synonym Full Names: resistin
• NCBI Official Symbol: RETN
• NCBI Official Synonym Symbols: ADSF; RSTN; XCP1; FIZZ3; RETN1
• NCBI Protein Information: resistin
• UniProt Protein Name: Resistin
• UniProt Gene Name: RETN
• UniProt Synonym Gene Names: FIZZ3; HXCP1; RSTN; ADSF
• UniProt Entry Name: RETN_HUMAN