Human resistin ELISA Kit | RETN elisa kit
human Resistin ELISA Kit
Gene Names
RETN; ADSF; RSTN; XCP1; FIZZ3; RETN1
Reactivity
Human
Synonyms
resistin; N/A; human Resistin ELISA Kit; human Resistin ELISA KIT; ADSF; FIZZ3; MGC126603; MGC126609; RETN1; RSTN; XCP1; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1; found in inflammatory zone 3; RETN elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human resistin. No significant cross-reactivity or interference between human resistin and analogues was observed.
Sequence Length
108
Samples
Serum, plasma and tissue homogenates
Assay Type
Sandwich
Detection Range
0.312 ng/ml -20 ng/ml.
Sensitivity
The minimum detectable dose of human resistin is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Related Product Information for RETN elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for resistin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any resistin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for resistin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of resistin bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,419 Da
NCBI Official Full Name
resistin
NCBI Official Synonym Full Names
resistin
NCBI Official Symbol
RETN
NCBI Official Synonym Symbols
ADSF; RSTN; XCP1; FIZZ3; RETN1
NCBI Protein Information
resistin; resistin delta2; found in inflammatory zone 3; cysteine-rich secreted protein FIZZ3; adipose tissue-specific secretory factor; cysteine-rich secreted protein A12-alpha-like 2; c/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1
UniProt Protein Name
Resistin
UniProt Gene Name
RETN
UniProt Synonym Gene Names
FIZZ3; HXCP1; RSTN; UNQ407/PRO1199; ADSF
UniProt Entry Name
RETN_HUMAN
