Standard Curve (Sample)
Mouse Resistin ELISA Kit | RETN elisa kit
Mouse Resistin PicoKine Fast ELISA Kit
Gene Names
Retn; ADSF; Rstn; Xcp4; Fizz3
Reactivity
Mouse
Synonyms
Resistin; N/A; Mouse Resistin PicoKine Fast ELISA Kit; Mouse Resistin; Resistin, isoform CRA_a; Retn; Xcp4; mCG_13014; RETN elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Retn
Sequence Length
114
Samples
Cell culture supernates, serum and plasma(heparin)
Detection Range
31.2pg/ml-2,000pg/ml
Sensitivity
<2pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for RETN elisa kit
Principle of the Assay: The Picokine™ Mouse Retn Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Retn with a 96-well strip plate that is pre-coated with antibody specific for Retn. The detection antibody is a biotinylated antibody specific for Retn. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Retn with immunogen: Expression system for standard: E.coli; Immunogen sequence: S21-S114. The kit is analytically validated with ready to use reagents. To measure Mouse Retn, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Mouse Retn in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Retn in the sample.!!Background/Introduction: Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance. Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin(for resistance to insulin), that may be the hormone potentially links obesity to diabetes.Resistin is overexpressed in mouse adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.The standard product used in this kit is mouse Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Resistin (18-day embryo whole body cDNA, RIKEN full-length enriched library, clone:1110029G18 product:resistin, full insert sequence)
NCBI Official Synonym Full Names
resistin
NCBI Official Symbol
Retn
NCBI Official Synonym Symbols
ADSF; Rstn; Xcp4; Fizz3
NCBI Protein Information
resistin
UniProt Protein Name
Resistin
UniProt Gene Name
Retn
UniProt Entry Name
Q5BMX4_MOUSE
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Product Notes
The Mouse RETN retn (Catalog #AAA125337) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA125337 ELISA Kit recognizes Mouse RETN. It is sometimes possible for the material contained within the vial of "Resistin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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