Application Data
Relaxin Recombinant Protein | RLN recombinant protein
Recombinant Relaxin (RLN)
Gene Names
RLN1; H1; H1RLX; RLXH1; bA12D24.3.1; bA12D24.3.2
Applications
Immunoprecipitation, Immunoprecipitation, ELISA, Western Blot, SDS-Page
Purity
>95%
Synonyms
Relaxin; N/A; Recombinant Relaxin (RLN); RLN recombinant protein
Host
E Coli
Purity/Purification
>95%
Form/Format
100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Applicable Applications for RLN recombinant protein
IP (CoImmunoprecipitation), IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE
Source
Prokaryotic expression.
Residues
Arg23~Cys186
Tags
N-terminal His-Tag
Tissue Specificity
Prostate.
Subcellular Location
Secreted.
Traits
Freeze-dried powder
Predicted isoelectric point
9.1
Predicted Molecular Mass
23.7kDa
Accurate Molecular Mass
20kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3.Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3.Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Application Data
Application Data
SDS-PAGE
SDS-PAGE
Sequence
Sequence
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
relaxin, partial
NCBI Official Synonym Full Names
relaxin 1
NCBI Official Symbol
RLN1
NCBI Official Synonym Symbols
H1; H1RLX; RLXH1; bA12D24.3.1; bA12D24.3.2
NCBI Protein Information
prorelaxin H1
UniProt Protein Name
Prorelaxin H1
UniProt Gene Name
RLN1
UniProt Entry Name
REL1_HUMAN
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Product Notes
The RLN rln1 (Catalog #AAA144363) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Relaxin can be used in a range of immunoassay formats including, but not limited to, IP (CoImmunoprecipitation), IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE. Researchers should empirically determine the suitability of the RLN rln1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Relaxin, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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