Negative Control RNA Control
siRNA Negative Control
Applications
RNA Interference (RNAi)
Synonyms
Negative Control; N/A; siRNA Negative Control; Negative Control rna control
Specificity
Negative Control Duplexes are ideal for use in RNA interference (RNAi) experiments as a control for sequence independent effects following siRNA delivery in any vertebrate cell line.
Form/Format
Lyophilized powder
Applicable Applications for Negative Control rna control
RNA Interference (RNAi)
Application Notes
It is recommended as a negative control for evaluating RNAi off-target effects, and in order to verify the accuracy of gene specific siRNA dependent RNAi.
Source
Synthetic
Direction for Use
Lab recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water.
Components
siRNA Negative Control is a non-targeting 21 nt siRNA designed.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Preparation and Storage
Store at -20°C for one year.
Related Product Information for Negative Control rna control
Negative Control for experiments using targeted siRNA transfection
Similar Products
Product Notes
The Negative Control (Catalog #AAA27802) is a RNA Control and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Negative Control can be used in a range of immunoassay formats including, but not limited to, RNA Interference (RNAi). It is recommended as a negative control for evaluating RNAi off-target effects, and in order to verify the accuracy of gene specific siRNA dependent RNAi. Researchers should empirically determine the suitability of the Negative Control for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Negative Control, RNA Control" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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