Application Data
(HTRF assay for RPS6KA3 / MAPKAPK1B activity 1 uM STK S1 substrate was incubated with different concentrations of RPS6KA3 / MAPKAPK1B protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
RPS6KA3/MAPKAPK1B recombinant protein
Recombinant RPS6KA3/MAPKAPK1B protein
Gene Names
RPS6KA3; CLS; RSK; HU-3; RSK2; MRX19; ISPK-1; p90-RSK2; pp90RSK2; MAPKAPK1B; S6K-alpha3
Synonyms
RPS6KA3/MAPKAPK1B; N/A; Recombinant RPS6KA3/MAPKAPK1B protein; RPS6KA3; ISPK1; MAPKAPK1B; RSK2; Ribosomal protein S6 kinase alpha-3; 90kD ribosomal protein S6 kinase 3; Insulin-stimulated protein kinase 1; MAP kinase-activated protein kinase 1b; Ribosomal S6 kinase 2; pp90RSK2; RPS6KA3/MAPKAPK1B recombinant protein
Host
Baculovirus
Form/Format
Recombinant RPS6KA3/MAPKAPK1B protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Protein Details
Recombinant RPS6KA3/MAPKAPK1B protein was expressed in a baculovirus expression system as the full length protein (accession number NP_004577.1) with a N-terminal DYKDDDDK tag.
Notes
This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(HTRF assay for RPS6KA3 / MAPKAPK1B activity 1 uM STK S1 substrate was incubated with different concentrations of RPS6KA3 / MAPKAPK1B protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for RPS6KA3 / MAPKAPK1B activity 1 uM STK S1 substrate was incubated with different concentrations of RPS6KA3 / MAPKAPK1B protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE
(Recombinant RPS6KA3 / MAPKAPK1B protein 10% SDS-PAGE with Coomassie blue staining MW: 84.7 kDa Purity: >95%)
SDS-PAGE
(Recombinant RPS6KA3 / MAPKAPK1B protein 10% SDS-PAGE with Coomassie blue staining MW: 84.7 kDa Purity: >95%)
Related Product Information for RPS6KA3/MAPKAPK1B recombinant protein
Background: RPS6KA3 also known as MAPKAPK1B is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signaling pathway. It acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RPS6KA3 also regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1.Diseases associated with RPS6KA3 include Coffin-Lowry Syndrome and Symptomatic Form Of Coffin-Lowry Syndrome In Female Carriers. Among its related pathways are Cellular Senescence (REACTOME) and Activated TLR4 signaling.
Product Categories/Family for RPS6KA3/MAPKAPK1B recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
740
NCBI Official Full Name
ribosomal protein S6 kinase alpha-3
NCBI Official Synonym Full Names
ribosomal protein S6 kinase, 90kDa, polypeptide 3
NCBI Official Symbol
RPS6KA3
NCBI Official Synonym Symbols
CLS; RSK; HU-3; RSK2; MRX19; ISPK-1; p90-RSK2; pp90RSK2; MAPKAPK1B; S6K-alpha3
NCBI Protein Information
ribosomal protein S6 kinase alpha-3; RSK-2; p90-RSK 3; MAPKAPK-1b; S6K-alpha-3; MAPKAP kinase 1b; ribosomal S6 kinase 2; MAPK-activated protein kinase 1b; insulin-stimulated protein kinase 1; MAP kinase-activated protein kinase 1b
UniProt Protein Name
Ribosomal protein S6 kinase alpha-3
UniProt Gene Name
RPS6KA3
UniProt Synonym Gene Names
ISPK1; MAPKAPK1B; RSK2; S6K-alpha-3; p90-RSK 3; p90RSK3; ISPK-1; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b; RSK-2
UniProt Entry Name
KS6A3_HUMAN
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Product Notes
The RPS6KA3/MAPKAPK1B rps6ka3 (Catalog #AAA60234) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "RPS6KA3/MAPKAPK1B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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