RSPO2 (L186P) Recombinant Protein | RSPO2 recombinant protein

Human RSPO2 (L186P) Protein (Fc Tag)

Cat. Num. AAA258273

• Gene Names: RSPO2; CRISTIN2
• Purity: >90% as determined by SDS-PAGE
• Synonyms: RSPO2 (L186P); N/A; Human RSPO2 (L186P) Protein (Fc Tag); RSPO2 Protein, Human, Recombinant (hFc Tag); R-spondin 2; CRISTIN2 Protein, Human; RSPO2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile Histidine, Arginine, 120mM NaCl, 0.02% Tween 80, 5% sucrose, pH6.0 Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence: Met1-Gly205

• Species: Human
• Endotoxin: <1.0 EU per ug protein as determined by the LAL method
• Predicted N Terminal: Gln 22
• Tag: C-human IgG1-Fc
• Activity: Measured by its ability to induce activation of betacatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 2-12ng/mL in the presence of 5ng/mL recombinant mouse Wnt3a.
• Protein Construction: A DNA sequence encoding the Human RSPO2 (Q6UXX9-1, with mutations L186P)(Met1-Gly205) was expressed with the Fc region of human IgG1 at the C-terminus.
• Preparation and Storage: Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Application Data

Application Data

(Measured by its ability to induce activation of betacatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 2-12ng/mL in the presence of 5ng/mL recombinant mouse Wnt3a.)

Related Product Information for RSPO2 recombinant protein

Background: R-spondin-2, also known as RSPO2, synergizes with Wnt to activate beta-catenin. RSPO2 is secreted proteins that regulate beta-catenin signaling. Activator of the beta-catenin signaling cascade leads to TCF-dependent gene activation. Action both in the canonical Wnt/beta- catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Probably also acts as a ligand for frizzled and LRP receptors. The encoding gene Rspo2 was identified as a novel common integration site for the mouse mammary tumor virus in viral induced mouse mammary tumors. Rspo2 and Rspo2/Wnt1 tumors contained many spindle cells, consistent with an epithelial-mesenchymal transformation phenotype. When Rspo2 and Rspo2/Wnt1 tumor cells were transferred into naive mice, they exhibited greater metastatic activity than cells derived from Wnt1 tumors.

References

Cadieu E, et al. (2009) Coat Variation in the Domestic Dog Is Governed by Variants in Three Genes. Science. 326: 150-153.
Kazanskaya O, et al. (2004) R-Spondin2 is a secreted activator of Wnt/beta-catenin signaling and is required for Xenopus myogenesis. Dev Cell. 7(4): 525-34.
Parker HG, et al. (2010) An insertion in the RSPO2 gene correlates with improper coat in the Portuguese water dog. J Hered. 101(5):612-7.

NCBI and Uniprot Product Information

• NCBI GI #: 222446611
• NCBI GeneID: 340419
• NCBI Accession #: NP_848660.3
• NCBI GenBank Nucleotide #: NM_178565.4
• UniProt Accession #: Q6UXX9; Q4G0U4; Q8N6X6; B3KVP0
• Molecular Weight: 22.0kD
• NCBI Official Full Name: R-spondin-2 isoform 1
• NCBI Official Synonym Full Names: R-spondin 2
• NCBI Official Symbol: RSPO2
• NCBI Official Synonym Symbols: CRISTIN2
• NCBI Protein Information: R-spondin-2; R-spondin 2 homolog; roof plate-specific spondin-2
• UniProt Protein Name: R-spondin-2
• UniProt Gene Name: RSPO2
• UniProt Synonym Gene Names: hRspo2
• UniProt Entry Name: RSPO2_HUMAN