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product-image-AAA112239_SC15.png Standard Curve (Sample)

Mouse R-spondin family, member 4 ELISA Kit | RSPO4 elisa kit

Mouse R-spondin-4, RSPO4 ELISA Kit

Gene Names
Rspo4; A730099F22; A930029K19Rik
Reactivity
Mouse
Synonyms
R-spondin family, member 4; N/A; Mouse R-spondin-4, RSPO4 ELISA Kit; Mouse R-spondin-4 (RSPO4) ELISA kit; RP5-824F16.1; C20orf182; CRISTIN4; FLJ16018; roof plate-specific spondin-4; R-spondin family; member 4; RSPO4 elisa kit
Ordering
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse RSPO4. No significant cross-reactivity or interference between mouse RSPO4 and analogues was observed.
Sequence Length
228
Samples
Serum, plasma, tissue homogenates.
Assay Type
Sandwich
Detection Range
34.4 pg/ml -2200 pg/ml.
Sensitivity
8.6 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

product-image-AAA112239_SC15.png Standard Curve (Sample)
Related Product Information for RSPO4 elisa kit
Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RSPO4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RSPO4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RSPO4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RSPO4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,866 Da
NCBI Official Full Name
R-spondin-4 isoform 1
NCBI Official Synonym Full Names
R-spondin family, member 4
NCBI Official Symbol
Rspo4
NCBI Official Synonym Symbols
A730099F22; A930029K19Rik
NCBI Protein Information
R-spondin-4; cristin-4; mCristin-4; R-spondin-like protein; roof plate-specific spondin-4; cysteine-rich and single thrombospondin domain-containing protein 4
UniProt Protein Name
R-spondin-4
UniProt Gene Name
Rspo4
UniProt Synonym Gene Names
Cristin-4; mCristin-4
UniProt Entry Name
RSPO4_MOUSE

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Product Notes

The Mouse RSPO4 rspo4 (Catalog #AAA112239) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA112239 ELISA Kit recognizes Mouse RSPO4. It is sometimes possible for the material contained within the vial of "R-spondin family, member 4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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