Application Data
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Prefusion glycoprotein F/RSV-preF ELISA Kit | RSV-F elisa kit
Human respiratory syncytial virus (RSV)(A2) Prefusion glycoprotein F/RSV-preF ELISA Kit
Reactivity
RSV
Synonyms
Prefusion glycoprotein F/RSV-preF; N/A; Human respiratory syncytial virus (RSV)(A2) Prefusion glycoprotein F/RSV-preF ELISA Kit; RSV-F elisa kit
Reactivity
RSV
Specificity
This assay recognizes recombinant Human respiratory syncytial virus (RSV)(A2) Prefusion glycoprotein F/RSV-preF.
Assay Type
Solid Phase Sandwich ELISA (quantitative)
Detection Range
1562.5-100000pg/ml
Sensitivity
202.4pg/mL
Notes
The kit has been verified by high purity Human respiratory syncytial virus (RSV)(A2) Prefusion glycoprotein F/RSV-preF Protein .
Preparation and Storage
Unopened Kit: Store at 2-8 degree C
Opened/Reconstituted Reagents: Please refer to CoA
This ELISA Kit is shipped at ambient temperature.
Opened/Reconstituted Reagents: Please refer to CoA
This ELISA Kit is shipped at ambient temperature.
Application Data
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Application Data
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Related Product Information for RSV-F elisa kit
This Human respiratory syncytial virus (RSV)(A2) Prefusion glycoprotein F/RSV-preF ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of RSV RSV Fusion protein in Recombinant protein. It contains recombinant RSV RSV Fusion, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. The G protein mediates attachment of the virus to cell surface receptors, while the F protein promotes fusion of the viral and cellular membranes, allowing entry of the virus ribonucleoprotein into the cell cytoplasm. The fusion (F) protein of RSV is synthesized as a nonfusogenic precursor protein (F), which during its migration to the cell surface is activated by cleavage into the disulfide-linked F1 and F2 subunits. This fusion is pH independent and occurs directly at the outer cell membrane, and the F2 subunit was identifed as the major determinant of RSV host cell specificity. The trimer of F1-F2 interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and induces the fusion between host cell and virion membranes. Notably, RSV fusion protein is unique in that it is able to interact directly with heparan sulfate and therefore is sufficient for virus infection. Furthermore, the fusion protein is also able to trigger p53-dependent apoptosis.
Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. The G protein mediates attachment of the virus to cell surface receptors, while the F protein promotes fusion of the viral and cellular membranes, allowing entry of the virus ribonucleoprotein into the cell cytoplasm. The fusion (F) protein of RSV is synthesized as a nonfusogenic precursor protein (F), which during its migration to the cell surface is activated by cleavage into the disulfide-linked F1 and F2 subunits. This fusion is pH independent and occurs directly at the outer cell membrane, and the F2 subunit was identifed as the major determinant of RSV host cell specificity. The trimer of F1-F2 interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and induces the fusion between host cell and virion membranes. Notably, RSV fusion protein is unique in that it is able to interact directly with heparan sulfate and therefore is sufficient for virus infection. Furthermore, the fusion protein is also able to trigger p53-dependent apoptosis.
References
Martin-Gallard. A. e. al.. 1993. . Ge. Virol. 7. (3). 453-8. Jos. . M. e. al.. 1997. . Ge. Virol. 78. 2411-8. Feldma. SA. e. al.. 1999. . Virol. 7. (8). 6610-7. Zlatev. K.T. e. al.. 2004. . Virol. 7. (9). 4675-83. Trent. A. e. al.. 2006. . Virol. 8. (2). 975-84. Braniga. . J. e. al.. 2006. . Ge. Virol. 8. (2). 395-8. Eckardt-Miche. J. e. al.. 2008. J. Virol. 82. 3236-49.
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Product Notes
The RSV RSV-F (Catalog #AAA259535) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA259535 ELISA Kit recognizes RSV RSV-F. It is sometimes possible for the material contained within the vial of "Prefusion glycoprotein F/RSV-preF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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