Standard Curve (Sample)
Rat runt-related transcription factor 2 ELISA Kit | RUNX2 elisa kit
Rat Runt-related transcription factor 2, RUNX2 ELISA Kit
Gene Names
Runx2; Cbfa1; OSF-2
Reactivity
Rat
Synonyms
runt-related transcription factor 2; N/A; Rat Runt-related transcription factor 2, RUNX2 ELISA Kit; Rat Runt-related transcription factor 2 (RUNX2) ELISA kit; RP1-166H4.1; AML3; CBFA1; CCD; CCD1; MGC120022; MGC120023; OSF2; PEA2aA; PEBP2A1; PEBP2A2; PEBP2aA; PEBP2aA1; CBF-alpha 1; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A; RUNX2 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat RUNX2. No significant cross-reactivity or interference between rat RUNX2 and analogues was observed.
Sequence Length
218
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
3.12 ng/ml-200 ng/ml.
Sensitivity
The minimum detectable dose of rat RUNX2 is typically less than 0.78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for RUNX2 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RUNX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RUNX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RUNX2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RUNX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
24,247 Da
NCBI Official Full Name
Runt-related transcription factor 2
NCBI Official Synonym Full Names
runt-related transcription factor 2
NCBI Official Symbol
Runx2
NCBI Official Synonym Symbols
Cbfa1; OSF-2
NCBI Protein Information
runt-related transcription factor 2; CBF-alpha-1; core-binding factor subunit alpha-1; runt related transcription factor 2; osteoblast-specific transcription factor 2
UniProt Protein Name
Runt-related transcription factor 2
UniProt Gene Name
Runx2
UniProt Synonym Gene Names
Cbfa1; Osf2; CBF-alpha-1; OSF-2
UniProt Entry Name
RUNX2_RAT
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Product Notes
The Rat RUNX2 runx2 (Catalog #AAA112302) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA112302 ELISA Kit recognizes Rat RUNX2. It is sometimes possible for the material contained within the vial of "runt-related transcription factor 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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