Mouse Soluble protein-100B ELISA Kit | S-100B elisa kit

Mouse Soluble protein-100B ELISA Kit

Cat. Num. AAA87191

• Gene Names: S100B; NEF; S100; S100-B; S100beta
• Reactivity: Mouse
• Synonyms: Soluble protein-100B; N/A; Mouse Soluble protein-100B ELISA Kit; Soluble protein-100B (Mouse); S-100B elisa kit

• Reactivity: Mouse
• Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma

• Preparation and Storage: Store all reagents at 2-8 degree C

Standard Curve (Sample)

Related Product Information for S-100B elisa kit

For Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma

Intended Uses: This S-100B ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of S-100B in the sample, this S-100B ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus S-100B concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of S-100B utilizes a multiclonal anti-S-100B antibody and an S-100B-HRP conjugate. The assay sample and buffer are incubated together with S-100B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the S-100B concentration since S-100B from samples and S-100B-HRP conjugate compete for the anti-S-100B antibody binding site. Since the number of sites is limited, as more sites are occupied by S-100B from the sample, fewer sites are left to bind S-100B-HRP conjugate. Standards of known S-100B concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of S-100B. The S-100B concentration in each sample is interpolated from this standard curve.

Product Categories/Family for S-100B elisa kit

Mouse ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 119629675
• NCBI GeneID: 6285
• UniProt Accession #: P04271; D3DSN6
• Molecular Weight: 10,713 Da
• NCBI Official Full Name: S100 calcium binding protein, beta (neural), isoform CRA_a
• NCBI Official Synonym Full Names: S100 calcium binding protein B
• NCBI Official Symbol: S100B
• NCBI Official Synonym Symbols: NEF; S100; S100-B; S100beta
• NCBI Protein Information: protein S100-B; OTTHUMP00000115888; S-100 protein subunit beta; S-100 calcium-binding protein, beta chain; S100 calcium-binding protein, beta (neural)
• UniProt Protein Name: Protein S100-B
• UniProt Gene Name: S100B
• UniProt Entry Name: S100B_HUMAN