Canine Substance P ELISA Kit | S.P elisa kit
Canine Substance P ELISA Kit
Gene Names
Tac1; TAC; PPTA3; Ppt5fl; RATPPTA3
Reactivity
Canine
Synonyms
Substance P; N/A; Canine Substance P ELISA Kit; S.P elisa kit
Reactivity
Canine
Specificity
This assay has high sensitivity and excellent specificity for detection of SP. No significant cross-reactivity or interference between SP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SP and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for S.P elisa kit
Intended Uses: This SP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of CanineSP. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: SP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SP antibody and an SP-HRP conjugate. The assay sample and buffer are incubated together with SP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SP concentration since SP from samples and SP-HRP conjugate compete for the anti-SP antibody binding site. Since the number of sites is limited, as more sites are occupied by SP from the sample, fewer sites are left to bind SP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SP concentration in each sample is interpolated from this standard curve.
Principle of the Assay: SP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SP antibody and an SP-HRP conjugate. The assay sample and buffer are incubated together with SP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SP concentration since SP from samples and SP-HRP conjugate compete for the anti-SP antibody binding site. Since the number of sites is limited, as more sites are occupied by SP from the sample, fewer sites are left to bind SP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SP concentration in each sample is interpolated from this standard curve.
Product Categories/Family for S.P elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
11,374 Da
NCBI Official Full Name
substance P
NCBI Official Synonym Full Names
tachykinin, precursor 1
NCBI Official Symbol
Tac1
NCBI Official Synonym Symbols
TAC; PPTA3; Ppt5fl; RATPPTA3
NCBI Protein Information
protachykinin-1; PPT; tachykinin 1; Tachykinin (substance P, neurokinin A, neuropeptide K, neuropeptide gamma)
UniProt Protein Name
Protachykinin-1
UniProt Gene Name
Tac1
UniProt Synonym Gene Names
Nka; Nkna; Tac2; NKA; NPK
UniProt Entry Name
TKN1_RAT
