Rat S100 Calcium Binding Protein A11 ELISA Kit | S100A11 elisa kit

Rat S100 Calcium Binding Protein A11 ELISA Kit

Cat. Num. AAA16592

• Gene Names: S100A11; MLN70; S100C; HEL-S-43
• Reactivity: Rat
• Synonyms: S100 Calcium Binding Protein A11; Rat S100 Calcium Binding Protein A11 ELISA Kit; S100A11 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of S100A11. No significant cross-reactivity or interference between S100A11 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between S100A11 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Highest Standards: 10ng/mL
• Concentration of Standards: Concentration of Standards
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for S100A11 elisa kit

Principle of the Assay: S100A11 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-S100A11 antibody and an S100A11-HRP conjugate. The assay sample and buffer are incubated together with S100A11-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the S100A11 concentration since S100A11 from samples and S100A11-HRP conjugate compete for the anti-S100A11 antibody binding site. Since the number of sites is limited, as more sites are occupied by S100A11 from the sample, fewer sites are left to bind S100A11-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100A11 concentration in each sample is interpolated from this standard curve.

Intended Uses: This S100A11 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat S100A11. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for S100A11 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 119573777
• NCBI GeneID: 6282
• Molecular Weight: 11,740 Da
• NCBI Official Full Name: S100 calcium binding protein A11 (calgizzarin)
• NCBI Official Synonym Full Names: S100 calcium binding protein A11
• NCBI Official Symbol: S100A11
• NCBI Official Synonym Symbols: MLN70; S100C; HEL-S-43
• NCBI Protein Information: protein S100-A11; MLN 70; calgizzarin; protein S100-C; epididymis secretory protein Li 43; metastatic lymph node gene 70 protein; S100 calcium-binding protein A11 (calgizzarin)
• UniProt Protein Name: Protein S100-A11
• UniProt Gene Name: S100A11
• UniProt Synonym Gene Names: MLN70; S100C; MLN 70
• UniProt Entry Name: S10AB_HUMAN