Human S100-A13 ELISA Kit | S100A13 elisa kit

Human S100-A13 ELISA Kit

Cat. Num. AAA263358

• Reactivity: Human
• Synonyms: S100-A13; N/A; Human S100-A13 ELISA Kit; Protein S100-A13; S100 calcium-binding protein A13; S100A13 elisa kit

• Reactivity: Human
• Specificity: The Human S100-A13 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human S100-A13 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
• Sequence Length: 98
• Samples: Culture Supernates, Serum, Plasma, Cell Lysates, Tissue Homogenates
• Sensitivity: < 7 pg/ml

• Detection Method: Colorimetric
• Preparation and Storage: Store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Standard Curve (Sample)

Related Product Information for S100A13 elisa kit

Background: S100A13 is an 11 kDa member of the S100 (soluble in 100% saturated ammonium sulfate) family of vertebrate EF-hand Ca2+-binding proteins. Like other S100 proteins, S100A13 is small and generally acidic, but contains a basic residue rich sequence at the C-terminus, and two EF hand motifs that bind with Ca2+ differing affinities. Some S100 proteins, including S100A13, are able to bind the cell surface receptor for advanced glycation endproducts (RAGE). Despite lacking a signal sequence, S100A13 plays an important role in Cu2+-dependent export of FGF-1 (FGF acidic) and IL-1 alpha from the cell in response to stresses such as heat shock, anoxia and starvation. Binding of copper is necessary for formation of a multiprotein complex between S100A13, FGF-1 and p40 synaptotagmin1 (syt1). Cu2+ ions supplied by S100A13 are thought to oxidize and downregulate the activity of FGF-1 prior to export. calcium influx may also play a similar role in FGF-1 release from neuronal cells. With FGF-1 and syt1, S100A13 likely perturbs the membrane, which allows the S100A13 protein complex to exit the cell. S100A13 has been proposed as a marker for angiogenesis in tumors and endometrium, due to its role in stress induced export of FGF-1.

Principle of the Assay: The Human S100-A13 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human S100-A13 in Cell Culture Supernates, Serum, Plasma, Cell Lysates, Tissue Homogenates. This assay employs an antibody specific for Human S100-A13 coated on a 96-well plate. Standards and samples are pipetted into the wells and S100-A13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human S100-A13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of S100-A13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 66737368
• NCBI GeneID: 6284
• NCBI Accession #: NP_001019381.1
• NCBI GenBank Nucleotide #: NM_001024210.1
• UniProt Accession #: Q99584; Q52PI9; Q6FGF8
• Molecular Weight: 11,471 Da
• NCBI Official Full Name: protein S100-A13
• NCBI Official Synonym Full Names: S100 calcium binding protein A13
• NCBI Official Symbol: S100A13
• NCBI Protein Information: protein S100-A13
• UniProt Protein Name: Protein S100-A13
• UniProt Gene Name: S100A13