Standard Curve (Sample)
(Mouse S100A9 PicoKine ELISA Kit standard curve)
Protein S100-A9 ELISA Kit | S100A9 elisa kit
Mouse S100A9 PicoKine ELISA Kit
Gene Names
S100a9; p14; Cagb; GAGB; L1Ag; BEE22; MRP14; 60B8Ag; AW546964
Reactivity
Mouse. There is no detectable cross-reactivity with other relevant proteins.
Synonyms
Protein S100-A9; N/A; Mouse S100A9 PicoKine ELISA Kit; Leukocyte L1 complex heavy chain; 60B8AG; CAGB; Calgranulin B; Calgranulin-B; Calprotectin L1H subunit; CFAG; CGLB; Cystic fibrosis antigen; Cystic fibrosis antigen B; HGNC:10499; L1AG; LIAG; MAC 387; MAC387; MIF; Migration inhibitory factor related protein 14; Migration inhibitory factor-related protein 14; MRP 14; MRP-14; MRP14; Myeloid-related protein 14; NIF; OTTHUMP00000015331; P14; S100 A9; S100 calcium binding protein A9; S100 calcium binding protein A9 calgranulin B; S100 calcium-binding protein A9; S100A9; S10A9_HUMAN; S100A9 elisa kit
Reactivity
Mouse. There is no detectable cross-reactivity with other relevant proteins.
Specificity
Natural and recombinant Mouse S100A9
Sequence Length
113
Samples
Cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA)
Assay Type
Quantitative Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Mouse S100A9 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Mouse S100A9 PicoKine ELISA Kit standard curve)
Related Product Information for S100A9 elisa kit
Principle of the Assay: Mouse S100A9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for S100A9 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: A2-K113) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for S100A9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse S100A9 amount of sample captured in plate.
Background/Introduction: Protein S100A9 also known as migration inhibitory factor-related protein 14 (MRP-14) or calgranulin- B is a protein that is encoded by the S100A9 gene. S100A9 is a member of the S100 family of proteins containing 2 EF hand calcium-binding motifs. It is mapped to Chromosome 3 in mouse. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in the inhibition of casein kinase. S100A9 complexes with S100A8, another member of the S100 family of calcium-modulated proteins regulates myeloid cell function by binding to Toll-like receptor-4 (TLR-4) and the receptor for advanced glycation end products.
Background/Introduction: Protein S100A9 also known as migration inhibitory factor-related protein 14 (MRP-14) or calgranulin- B is a protein that is encoded by the S100A9 gene. S100A9 is a member of the S100 family of proteins containing 2 EF hand calcium-binding motifs. It is mapped to Chromosome 3 in mouse. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in the inhibition of casein kinase. S100A9 complexes with S100A8, another member of the S100 family of calcium-modulated proteins regulates myeloid cell function by binding to Toll-like receptor-4 (TLR-4) and the receptor for advanced glycation end products.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,049 Da
NCBI Official Full Name
protein S100-A9
NCBI Official Synonym Full Names
S100 calcium binding protein A9 (calgranulin B)
NCBI Official Symbol
S100a9
NCBI Official Synonym Symbols
p14; Cagb; GAGB; L1Ag; BEE22; MRP14; 60B8Ag; AW546964
NCBI Protein Information
protein S100-A9; S100 calcium-binding protein A9; leukocyte L1 complex heavy chain; migration inhibitory factor-related protein 14
UniProt Protein Name
Protein S100-A9
UniProt Gene Name
S100a9
UniProt Synonym Gene Names
Cagb; Mrp14; MRP-14; p14
UniProt Entry Name
S10A9_MOUSE
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Product Notes
The Mouse. There is no detectable cross-reactivity with other relevant proteins. S100A9 s100a9 (Catalog #AAA46121) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA46121 ELISA Kit recognizes Mouse. There is no detectable cross-reactivity with other relevant proteins. S100A9. It is sometimes possible for the material contained within the vial of "Protein S100-A9, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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